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Multifunctional Bioinstructive 3D Architectures to Modulate Cellular Behavior

Vaithilingam, Jayasheelan; Sanjuan?Alberte, Paola; Campora, Simona; Rance, Graham A.; Jiang, Long; Thorpe, Jordan; Burroughs, Laurence; Tuck, Christopher J.; Denning, Chris; Wildman, Ricky D.; Hague, Richard J. M.; Alexander, Morgan R.; Rawson, Frankie J.

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Authors

Jayasheelan Vaithilingam

Paola Sanjuan?Alberte

Simona Campora

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GRAHAM RANCE Graham.Rance@nottingham.ac.uk
Senior Research Fellow

LONG JIANG LONG.JIANG@NOTTINGHAM.AC.UK
Surface Analytical Officer

Jordan Thorpe

Laurence Burroughs

CHRISTOPHER TUCK CHRISTOPHER.TUCK@NOTTINGHAM.AC.UK
Professor of Materials Engineering

CHRIS DENNING chris.denning@nottingham.ac.uk
Professor of Stem Cell Biology

RICKY WILDMAN RICKY.WILDMAN@NOTTINGHAM.AC.UK
Professor of Multiphase Flow and Mechanics

RICHARD HAGUE RICHARD.HAGUE@NOTTINGHAM.AC.UK
Professor of Additive Manufacturing

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MORGAN ALEXANDER MORGAN.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Biomedical Surfaces



Abstract

Biological structures control cell behavior via physical, chemical, electrical, and mechanical cues. Approaches that allow us to build devices that mimic these cues in a combinatorial way are lacking due to there being no suitable instructive materials and limited manufacturing procedures. This challenge is addressed by developing a new conductive composite material, allowing for the fabrication of 3D biomimetic structures in a single manufacturing method based on two?photon polymerization. The approach induces a combinatorial biostimulative input that can be tailored to a specific application. Development of the 3D architecture is performed with a chemically actuating photocurable acrylate previously identified for cardiomyocyte attachment. The material is made conductive by impregnation with multiwalled carbon nanotubes. The bioinstructive effect of 3D nano? and microtopography is combined with electrical stimulation, incorporating biochemical, and electromechanical cues to stimulate cells in serum?free media. The manufactured architecture is combined with cardiomyocytes derived from human pluripotent stem cells. It is demonstrated that by mimicking biological occurring cues, cardiomyocyte behavior can be modulated. This represents a step change in the ability to manufacture 3D multifunctional biomimetic modulatory architectures. This platform technology has implications in areas spanning regenerative medicine, tissue engineering to biosensing, and may lead to more accurate models for predicting toxicity.

Citation

Vaithilingam, J., Sanjuan‐Alberte, P., Campora, S., Rance, G. A., Jiang, L., Thorpe, J., …Rawson, F. J. (2019). Multifunctional Bioinstructive 3D Architectures to Modulate Cellular Behavior. Advanced Functional Materials, 29(38), Article 1902016. https://doi.org/10.1002/adfm.201902016

Journal Article Type Article
Acceptance Date Jul 6, 2019
Online Publication Date Jul 28, 2019
Publication Date Sep 19, 2019
Deposit Date Oct 8, 2020
Publicly Available Date Mar 29, 2024
Journal Advanced Functional Materials
Print ISSN 1616-301X
Electronic ISSN 1616-3028
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 29
Issue 38
Article Number 1902016
DOI https://doi.org/10.1002/adfm.201902016
Keywords Electrochemistry; Electronic, Optical and magnetic materials; General chemical engineering; Condensed matter physics; Biomaterials
Public URL https://nottingham-repository.worktribe.com/output/2469370
Publisher URL https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.201902016

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