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Wilms' Tumor protein 1 and enzymatic oxidation of 5-methylcytosine in brain tumors: potential perspectives (2018)
Journal Article
Ramsawhook, A., Ruzov, A., & Coyle, B. (2018). Wilms' Tumor protein 1 and enzymatic oxidation of 5-methylcytosine in brain tumors: potential perspectives. Frontiers in Cell and Developmental Biology, 6, doi:10.3389/fcell.2018.00026. ISSN 2296-634X

The patterns of 5-methylcytosine (5mC) and its oxidized derivatives, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) are reportedly altered in a range of cancers. Likewise, Wilms’ Tumor protein 1 (WT1), a transcription factor... Read More

A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells (2018)
Journal Article
Ahmed, E. M., Bandopadhyay, G., Coyle, B., & Grabowska, A. M. (2018). A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells. Cellular Oncology, 41(3), doi:10.1007/s13402-018-0374-8. ISSN 2211-3436

Purpose Glioblastoma Multiforme (GBM) is the commonest brain tumour in adults. A population of cells, known as cancer stem cells (CSCs), is thought to mediate chemo/radiotherapy resistance. CD133 is a cell surface marker to identify and isolate... Read More

Immunostaining for DNA modifications: computational analysis of confocal images (2017)
Journal Article
Ramsawhook, A., Lewis, L. C., Eleftheriou, M., Abakir, A., Durczak, P. M., Markus, R., …Ruzov, A. (2017). Immunostaining for DNA modifications: computational analysis of confocal images. Journal of Visualized Experiments, doi:10.3791/56318. ISSN 1940-087X

For several decades, 5-methylcytosine (5mC) has been thought to be the only DNA modification with a functional significance in metazoans. The discovery of enzymatic oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carb... Read More

Medulloblastoma and ependymoma cells display levels of 5-carboxylcytosine and elevated TET1 expression (2017)
Journal Article
Ramsawhook, A., Lewis, L., Coyle, B., & Ruzov, A. (2017). Medulloblastoma and ependymoma cells display levels of 5-carboxylcytosine and elevated TET1 expression. Clinical Epigenetics, 9(18), doi:10.1186/s13148-016-0306-2. ISSN 1868-7075

Background Alteration of DNA methylation (5-methylcytosine, 5mC) patterns represents one of the causes of tumorigenesis and cancer progression. Tet proteins can oxidize 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine and 5-carboxylcytosine (... Read More

In vitro models of medulloblastoma: choosing the right tool for the job (2016)
Journal Article
Ivanov, D. P., Coyle, B., Walker, D. A., & Grabowska, A. M. (2016). In vitro models of medulloblastoma: choosing the right tool for the job. Journal of Biotechnology, 236, doi:10.1016/j.jbiotec.2016.07.028. ISSN 0168-1656

The recently-defined four molecular subgroups of medulloblastoma have required updating of our understanding of in vitro models to include molecular classification and risk stratification features from clinical practice. This review seeks to build a... Read More

Long-term exposure to irinotecan reduces cell migration in glioma cells. (2016)
Journal Article
Al-Ghafari, A. B., Punjaruk, W., Storer, L., Carrier, D., Hussein, D., Coyle, B., & Kerr, I. D. (2016). Long-term exposure to irinotecan reduces cell migration in glioma cells. Journal of Neuro-Oncology, 127(3), doi:10.1007/s11060-016-2058-4. ISSN 0167-594X

In spite of considerable research into the therapies for glioblastoma multiforme this tumour type remains very difficult to treat. As well as having a tendency to be inherently resistant to chemotherapy, glioblastoma multiforme also displays local in... Read More

CD105 (Endoglin) exerts prognostic effects via its role in the microvascular niche of paediatric high grade glioma (2012)
Journal Article
in the microvascular niche of paediatric high grade glioma. Acta Neuropathologica, 124(1), doi:10.1007/s00401-012-0952-1. ISSN 0001-6322

Paediatric high grade glioma (pHGG) (World Health Organisation astrocytoma grades III and IV) remains poor prognosis tumours, with a median survival of only 15 months following diagnosis. Current investigation of anti-angiogenic strategies has focuse... Read More

Pediatric brain tumor cancer stem cells: cell cycle dynamics, DNA repair, and etoposide extrusion (2010)
Journal Article
Hussein, D., Punjaruk, W., Storer, L. C. D., Shaw, L., Othman, R. T., Peet, A., …Coyle, B. (2011). Pediatric brain tumor cancer stem cells: cell cycle dynamics, DNA repair, and etoposide extrusion. Neuro-Oncology, 13(1), 70-83. doi:10.1093/neuonc/noq144

Reliable model systems are needed to elucidate the role cancer stem cells (CSCs) play in pediatric brain tumor drug resistance. The majority of studies to date have focused on clinically distinct adult tumors and restricted tumor types. Here, the CSC... Read More