A Thermoresponsive and Magnetic Colloid for 3D Cell Expansion and Reconfiguration

Saunders, Brian R.; Gould, Toby; Saeed, Aram Omer; Francini, Nora; White, Lisa; Dixon, James E.; Gould, Toby W.A.; Rashidi, Hassan; Al Ghanami, Racha Cheikh; Hruschka, Veronika; Redl, Heinz; Saunders, Brian R; Alexander, Cameron; Shakesheff, Kevin M.

Abstract

A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.

Citation

Saunders, B. R., Gould, T., Saeed, A. O., Francini, N., White, L., Dixon, J. E., …Shakesheff, K. M. (2015). A Thermoresponsive and Magnetic Colloid for 3D Cell Expansion and Reconfiguration. Advanced Materials, 27(4), 662-668. https://doi.org/10.1002/adma.201403626

• Journal Article Type: Article
• Online Publication Date: Nov 29, 2014
• Publication Date: 2015-01
• Deposit Date: Jun 12, 2015
• Publicly Available Date: Jun 12, 2015
• Journal: Advanced Materials
• Print ISSN: 0935-9648
• Electronic ISSN: 1521-4095
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 4
• Pages: 662-668
• DOI: https://doi.org/10.1002/adma.201403626
• Keywords: responsive polymers, tissue engineering, regenerative medicine, stem cell culture
• Public URL: https://nottingham-repository.worktribe.com/output/992989
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/adma.201403626/full
• Contract Date: Jun 12, 2015