Skip to main content

Research Repository

Advanced Search

Association between maternal micronutrient status, oxidative stress and common genetic variants in antioxidant enzymes at 15 weeks’ gestation in nulliparous women who subsequently develop pre-eclampsia

Mistry, Hiten D.; Gill, Carolyn; Kurlak, L.O.; Seed, Paul T.; Hestketh, John; Meplan, Catherine; Schomburg, Lutz; Chappell, Lucy C.; Morgan, Linda; Poston, Lucilla

Authors

Hiten D. Mistry

Carolyn Gill

L.O. Kurlak

Paul T. Seed

John Hestketh

Catherine Meplan

Lutz Schomburg

Lucy C. Chappell

Linda Morgan

Lucilla Poston



Abstract

Aims: Pre-eclampsia is a pregnancy-specific condition affecting 2-7% of women and a leading cause of perinatal and maternal morbidity and mortality. Deficiencies of specific micronutrient antioxidant activities associated with copper, selenium, zinc and manganese, have previously been linked to pre-eclampsia at time of disease. Our aims were to investigate whether maternal plasma micronutrient concentrations and related antioxidant enzyme activities are altered prior to pre-eclampsia onset and to examine the dependence on genetic variations in these antioxidant enzymes.
Methods: Pre-disease plasma samples (15+1 weeks’ gestation) were obtained from women enrolled in the international SCreening fOr Pregnancy Endpoints (SCOPE) study who subsequently developed pre-eclampsia (n=244), and age- and BMI-matched normotensive controls (n=472). Micronutrient concentrations were measured by inductively coupled plasma mass spectrometry; associated antioxidant enzyme activities, selenoprotein-P, caeruloplasmin concentrations and activities, antioxidant capacity and markers of oxidative stress were measured by colorimetric assays. Sixty four tagSNPs within genes encoding the antioxidant enzymes and selenoprotein-P were genotyped using allele-specific competitive PCR.
Results: Plasma copper and caeruloplasmin concentrations were modestly, but significantly elevated in women who subsequently developed pre-eclampsia (both P

Citation

Mistry, H. D., Gill, C., Kurlak, L., Seed, P. T., Hestketh, J., Meplan, C., …Poston, L. (2015). Association between maternal micronutrient status, oxidative stress and common genetic variants in antioxidant enzymes at 15 weeks’ gestation in nulliparous women who subsequently develop pre-eclampsia. Free Radical Biology and Medicine, 78, https://doi.org/10.1016/j.freeradbiomed.2014.10.580

Journal Article Type Article
Acceptance Date Oct 29, 2014
Online Publication Date Nov 6, 2014
Publication Date Jan 1, 2015
Deposit Date Jul 21, 2017
Publicly Available Date Mar 29, 2024
Journal Free Radical Biology and Medicine
Print ISSN 0891-5849
Electronic ISSN 1873-4596
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 78
DOI https://doi.org/10.1016/j.freeradbiomed.2014.10.580
Keywords Micronutrients; Preeclampsia; Hypertension; Pregnancy; Antioxidants; Oxidative stress; Free radicals
Public URL https://nottingham-repository.worktribe.com/output/985417
Publisher URL http://www.sciencedirect.com/science/article/pii/S0891584914010818

Files





You might also like



Downloadable Citations