Hiten D. Mistry
Association between maternal micronutrient status, oxidative stress and common genetic variants in antioxidant enzymes at 15 weeks’ gestation in nulliparous women who subsequently develop pre-eclampsia
Mistry, Hiten D.; Gill, Carolyn; Kurlak, L.O.; Seed, Paul T.; Hestketh, John; Meplan, Catherine; Schomburg, Lutz; Chappell, Lucy C.; Morgan, Linda; Poston, Lucilla
Authors
Carolyn Gill
L.O. Kurlak
Paul T. Seed
John Hestketh
Catherine Meplan
Lutz Schomburg
Lucy C. Chappell
Linda Morgan
Lucilla Poston
Abstract
Aims: Pre-eclampsia is a pregnancy-specific condition affecting 2-7% of women and a leading cause of perinatal and maternal morbidity and mortality. Deficiencies of specific micronutrient antioxidant activities associated with copper, selenium, zinc and manganese, have previously been linked to pre-eclampsia at time of disease. Our aims were to investigate whether maternal plasma micronutrient concentrations and related antioxidant enzyme activities are altered prior to pre-eclampsia onset and to examine the dependence on genetic variations in these antioxidant enzymes.
Methods: Pre-disease plasma samples (15+1 weeks’ gestation) were obtained from women enrolled in the international SCreening fOr Pregnancy Endpoints (SCOPE) study who subsequently developed pre-eclampsia (n=244), and age- and BMI-matched normotensive controls (n=472). Micronutrient concentrations were measured by inductively coupled plasma mass spectrometry; associated antioxidant enzyme activities, selenoprotein-P, caeruloplasmin concentrations and activities, antioxidant capacity and markers of oxidative stress were measured by colorimetric assays. Sixty four tagSNPs within genes encoding the antioxidant enzymes and selenoprotein-P were genotyped using allele-specific competitive PCR.
Results: Plasma copper and caeruloplasmin concentrations were modestly, but significantly elevated in women who subsequently developed pre-eclampsia (both P
Citation
Mistry, H. D., Gill, C., Kurlak, L., Seed, P. T., Hestketh, J., Meplan, C., …Poston, L. (2015). Association between maternal micronutrient status, oxidative stress and common genetic variants in antioxidant enzymes at 15 weeks’ gestation in nulliparous women who subsequently develop pre-eclampsia. Free Radical Biology and Medicine, 78, https://doi.org/10.1016/j.freeradbiomed.2014.10.580
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 29, 2014 |
Online Publication Date | Nov 6, 2014 |
Publication Date | Jan 1, 2015 |
Deposit Date | Jul 21, 2017 |
Publicly Available Date | Jul 21, 2017 |
Journal | Free Radical Biology and Medicine |
Print ISSN | 0891-5849 |
Electronic ISSN | 1873-4596 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 78 |
DOI | https://doi.org/10.1016/j.freeradbiomed.2014.10.580 |
Keywords | Micronutrients; Preeclampsia; Hypertension; Pregnancy; Antioxidants; Oxidative stress; Free radicals |
Public URL | https://nottingham-repository.worktribe.com/output/985417 |
Publisher URL | http://www.sciencedirect.com/science/article/pii/S0891584914010818 |
Contract Date | Jul 21, 2017 |
Files
1-s2.0-S0891584914010818-main.pdf
(321 Kb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search