Lina A. Dahabiyeh
A pilot study of alterations in oxidized angiotensinogen and antioxidants in pre-eclamptic pregnancy
Dahabiyeh, Lina A.; Tooth, David; Kurlak, Lesia O.; Mistry, Hiten D.; Pipkin, Fiona Broughton; Barrett, David A.
Authors
David Tooth
Lesia O. Kurlak
Hiten D. Mistry
Fiona Broughton Pipkin
David A. Barrett
Abstract
© 2020, The Author(s). The oxidation status of angiotensinogen (AGT) may have a critical role in pre-eclampsia. We used a validated, quantitative, mass spectrometry-based method to measure the oxidized and total AGT levels in plasma of pre-eclamptic women (n = 17), normotensive-matched controls (n = 17), and healthy non-pregnant women (n = 10). Measurements of plasma glutathione peroxidase (GPx) activity and serum selenium concentrations were performed as markers of circulating antioxidant capacity. Higher proportions of oxidized AGT in plasma from pre-eclamptic women compared to matched normotensive pregnant controls (P = 0.006), whilst maintaining a similar total plasma AGT concentration were found. In the pre-eclamptic group, blood pressure were correlated with the proportion of oxidized AGT; no such correlation was seen in the normotensive pregnant women. Plasma GPx was inversely correlated with oxidized AGT, and there was an inverse association between serum selenium concentration and the proportion of oxidized AGT. This is the first time that oxidized AGT in human plasma has been linked directly to antioxidant status, providing a mechanism for the enhanced oxidative stress in pre-eclampsia. We now provide pathophysiological evidence that the conversion of the reduced form of AGT to its more active oxidized form is associated with inadequate antioxidant status and could indeed contribute to the hypertension of pre-eclampsia.
Citation
Dahabiyeh, L. A., Tooth, D., Kurlak, L. O., Mistry, H. D., Pipkin, F. B., & Barrett, D. A. (2020). A pilot study of alterations in oxidized angiotensinogen and antioxidants in pre-eclamptic pregnancy. Scientific Reports, 10(1), Article 1956. https://doi.org/10.1038/s41598-020-58930-7
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 20, 2020 |
Online Publication Date | Feb 6, 2020 |
Publication Date | Feb 6, 2020 |
Deposit Date | Jan 21, 2020 |
Publicly Available Date | Feb 10, 2020 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 1 |
Article Number | 1956 |
DOI | https://doi.org/10.1038/s41598-020-58930-7 |
Keywords | Multidisciplinary |
Public URL | https://nottingham-repository.worktribe.com/output/3774773 |
Publisher URL | https://www.nature.com/articles/s41598-020-58930-7 |
Additional Information | Received: 20 November 2019; Accepted: 20 January 2020; First Online: 6 February 2020; : The authors declare no competing interests. |
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