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A pilot study of alterations in oxidized angiotensinogen and antioxidants in pre-eclamptic pregnancy

Dahabiyeh, Lina A.; Tooth, David; Kurlak, Lesia O.; Mistry, Hiten D.; Pipkin, Fiona Broughton; Barrett, David A.


Lina A. Dahabiyeh

David Tooth

Lesia O. Kurlak

Hiten D. Mistry

Fiona Broughton Pipkin

David A. Barrett


© 2020, The Author(s). The oxidation status of angiotensinogen (AGT) may have a critical role in pre-eclampsia. We used a validated, quantitative, mass spectrometry-based method to measure the oxidized and total AGT levels in plasma of pre-eclamptic women (n = 17), normotensive-matched controls (n = 17), and healthy non-pregnant women (n = 10). Measurements of plasma glutathione peroxidase (GPx) activity and serum selenium concentrations were performed as markers of circulating antioxidant capacity. Higher proportions of oxidized AGT in plasma from pre-eclamptic women compared to matched normotensive pregnant controls (P = 0.006), whilst maintaining a similar total plasma AGT concentration were found. In the pre-eclamptic group, blood pressure were correlated with the proportion of oxidized AGT; no such correlation was seen in the normotensive pregnant women. Plasma GPx was inversely correlated with oxidized AGT, and there was an inverse association between serum selenium concentration and the proportion of oxidized AGT. This is the first time that oxidized AGT in human plasma has been linked directly to antioxidant status, providing a mechanism for the enhanced oxidative stress in pre-eclampsia. We now provide pathophysiological evidence that the conversion of the reduced form of AGT to its more active oxidized form is associated with inadequate antioxidant status and could indeed contribute to the hypertension of pre-eclampsia.


Dahabiyeh, L. A., Tooth, D., Kurlak, L. O., Mistry, H. D., Pipkin, F. B., & Barrett, D. A. (2020). A pilot study of alterations in oxidized angiotensinogen and antioxidants in pre-eclamptic pregnancy. Scientific Reports, 10(1),

Journal Article Type Article
Acceptance Date Jan 20, 2020
Online Publication Date Feb 6, 2020
Publication Date Feb 6, 2020
Deposit Date Jan 21, 2020
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
Article Number 1956
Keywords Multidisciplinary
Public URL
Publisher URL
Additional Information Received: 20 November 2019; Accepted: 20 January 2020; First Online: 6 February 2020; : The authors declare no competing interests.


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