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Adventitious rooting declines with the vegetative to reproductive switch and involves a changed auxin homeostasis

Rasmussen, Amanda; Hosseini, Seyed Abdollah; Hajirezaei, Mohammed-Reza; Druege, Uwe; Geelen, Danny

Authors

Seyed Abdollah Hosseini

Mohammed-Reza Hajirezaei

Uwe Druege

Danny Geelen



Abstract

Adventitious rooting, whereby roots form from non-root tissues, is critical to the forestry and horticultural industries that depend on propagating plants from cuttings. A major problem is that age of the tissue affects the ability of the cutting to form adventitious roots. Here, a model system has been developed using Pisum sativum to differentiate between different interpretations of ageing. It is shown that the decline in adventitious rooting is linked to the ontogenetic switch from vegetative to floral and is mainly attributed to the cutting base. Using rms mutants it is demonstrated that the decline is not a result of increased strigolactones inhibiting adventitious root formation. Monitoring endogenous levels of a range of other hormones including a range of cytokinins in the rooting zone revealed that a peak in jasmonic acid is delayed in cuttings from floral plants. Additionally, there is an early peak in indole-3-acetic acid levels 6h post excision in cuttings from vegetative plants, which is absent in cuttings from floral plants. These results were confirmed using DR5:GUS expression. Exogenous supplementation of young cuttings with either jasmonic acid or indole-3-acetic acid promoted adventitious rooting, but neither of these hormones was able to promote adventitious rooting in mature cuttings. DR5:GUS expression was observed to increase in juvenile cuttings with increasing auxin treatment but not in the mature cuttings. Therefore, it seems the vegetative to floral ontogenetic switch involves an alteration in the tissue’s auxin homeostasis that significantly reduces the indole-3-acetic acid pool and ultimately results in a decline in adventitious root formation.

Citation

Rasmussen, A., Hosseini, S. A., Hajirezaei, M., Druege, U., & Geelen, D. (2015). Adventitious rooting declines with the vegetative to reproductive switch and involves a changed auxin homeostasis. Journal of Experimental Botany, 65(4), https://doi.org/10.1093/jxb/eru499

Journal Article Type Article
Acceptance Date Nov 24, 2014
Online Publication Date Dec 24, 2014
Publication Date Mar 1, 2015
Deposit Date Jan 25, 2017
Publicly Available Date Jan 25, 2017
Journal Journal of Experimental Botany
Print ISSN 0022-0957
Electronic ISSN 1460-2431
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 65
Issue 4
DOI https://doi.org/10.1093/jxb/eru499
Keywords Adventitious roots, auxin, cytokinin, flowering induction, jasmonic acid, maturation, strigolactone
Public URL http://eprints.nottingham.ac.uk/id/eprint/40082
Publisher URL https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eru499
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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