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Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells

Sidney, Laura E.; McIntosh, Owen D.; Hopkinson, Andrew

Authors

Owen D. McIntosh owen.mcintosh@nottingham.ac.uk

Andrew Hopkinson andrew.hopkinson@nottingham.ac.uk



Abstract

Purpose: Cells of the corneal epithelium and stroma can be distinguished in vivo by different intermediate filaments, cytokeratins for corneal epithelial cells (CEC) and vimentin for keratocytes. Isolated and cultured keratocytes change phenotype, losing expression of keratocyte markers and gaining markers associated with mesenchymal stromal cells (MSC). This study investigates this change in phenotype in relation to intermediate filament expression in cultured corneal stromal cells (CSC) compared to CEC.

Methods: Expression of epithelial markers (CK3, CK12, CK19, pan cytokeratin, E-cadherin), keratocyte markers (CD34, vimentin) and MSC markers (CD73, CD90 and CD105) were compared in CEC and CSC by immunocytochemistry and RT-qPCR. Expression was evaluated at different stages of CSC culture and compared to another stromal cell type, extracted from Wharton’s jelly (WJ-MSC).


Results: In vivo keratocytes did not express cytokeratins. However, cultured CSC expressed epithelial-associated CK3, CK12 and CK19 but other cytokeratins. Expression of cytokeratins increased as CSC were passaged and decreased as CSC were induced to become quiescent. Comparatively, WJ-MSC, expressed lower levels of CK3, CK12 and CK19, but also stained for pan cytokeratin and expressed KRT5.


Conclusions: Cultured CSC undergo phenotypic change during culture, expressing specific cytokeratin filaments normally associated with CEC. Cytokeratin expression begins as cells are cultured on plastic and increases with passage. This discovery may influence the way that differences are discerned between cultured CEC and CSC. Investigators need to be aware that the expression of cytokeratins does not necessarily represent epithelial contamination, and that CEC and CSC may be more related than previously recognised.

Journal Article Type Article
Publication Date Nov 1, 2015
Journal Investigative Ophthalmology and Visual Science
Print ISSN 0146-0404
Electronic ISSN 1552-5783
Publisher Association for Research in Vision and Ophthalmology
Peer Reviewed Peer Reviewed
Volume 56
Issue 12
APA6 Citation Sidney, L. E., McIntosh, O. D., & Hopkinson, A. (2015). Phenotypic change and induction of cytokeratin expression during in vitro culture of corneal stromal cells. Investigative Ophthalmology & Visual Science, 56(12), https://doi.org/10.1167/iovs.15-17810
DOI https://doi.org/10.1167/iovs.15-17810
Keywords Cornea, Corneal stromal cells, Cytokeratins, Corneal epithelial cells, Mesenchymal stem cells
Publisher URL http://iovs.arvojournals.org/article.aspx?articleid=2469548
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Sidney et al. Cytokeratin Expression in Stromal Cells.pdf (4 Mb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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