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Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans

Gillis, Richard B.; Adams, Gary G.; Besong, David T.M.; Machová, Eva; Ebringerová, Anna; Rowe, Arthur J.; Harding, Stephen E.; Patel, Trushar R.

Authors

Gary G. Adams

David T.M. Besong

Eva Machová

Anna Ebringerová

Arthur J. Rowe

Trushar R. Patel



Abstract

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins).

Citation

Gillis, R. B., Adams, G. G., Besong, D. T., Machová, E., Ebringerová, A., Rowe, A. J., …Patel, T. R. (2017). Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans. European Biophysics Journal, 46(3), 235-245. https://doi.org/10.1007/s00249-016-1159-5

Journal Article Type Article
Acceptance Date Jul 11, 2016
Online Publication Date Jul 21, 2016
Publication Date Apr 1, 2017
Deposit Date Apr 3, 2017
Publicly Available Date Apr 3, 2017
Journal European Biophysics Journal
Print ISSN 0175-7571
Electronic ISSN 0175-7571
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 46
Issue 3
Pages 235-245
DOI https://doi.org/10.1007/s00249-016-1159-5
Keywords AUC; Extended Fujita approach; MULTISIG; SEDFIT-MSTAR; Sedimentation
Public URL http://eprints.nottingham.ac.uk/id/eprint/41686
Publisher URL http://link.springer.com/article/10.1007%2Fs00249-016-1159-5
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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