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An in vitro model for assessing effective scrapie decontamination

Gough, Kevin C.; Baker, C.A.; Maddison, B.C.

Authors

KEVIN GOUGH kevin.gough@nottingham.ac.uk
Professor of Biochemistry and Pathology

C.A. Baker

B.C. Maddison



Abstract

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity within the farm is not adequately removed by current recommended guidelines for farm decontamination. We describe an in vitro method for modelling decontamination, specifically the removal of scrapie prions from the surface of concrete fomites within buildings that have housed scrapie infected animals. Concrete that had been spiked with low amounts of a diluted scrapie positive brain homogenate was sampled before and after decontamination. Extracts were used to seed a semi-quantitative serial protein misfolding cyclic amplification assay (sPMCA). We demonstrate that methods currently recommended for prion decontamination result in inadequate reduction of prion seeding activity within this in vitro assay. Effective treatment was achieved using repeat dosing of surfaces with 20,000 ppm available chlorine for 4 h.

Citation

Gough, K. C., Baker, C., & Maddison, B. (2017). An in vitro model for assessing effective scrapie decontamination. Veterinary Microbiology, 207, https://doi.org/10.1016/j.vetmic.2017.05.018

Journal Article Type Article
Acceptance Date May 20, 2017
Online Publication Date May 27, 2017
Publication Date Aug 1, 2017
Deposit Date Jul 27, 2017
Publicly Available Date Jul 27, 2017
Journal Veterinary Microbiology
Print ISSN 0378-1135
Electronic ISSN 1873-2542
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 207
DOI https://doi.org/10.1016/j.vetmic.2017.05.018
Keywords Scrapie; Prion; sPMCA; Decontamination; Fomite
Public URL http://eprints.nottingham.ac.uk/id/eprint/44469
Publisher URL http://www.sciencedirect.com/science/article/pii/S037811351730202X
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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