MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans
Khalaf, Asseel; Hoad, Caroline; Menys, Alex; Nowak, Adam; Taylor, Stuart A.; Paparo, Stephen; Lingaya, Melanie; Falcone, Yirga; Singh, Gulzar; Spiller, Robin C.; Gowland, Penny A.; Marciani, Luca; Moran, Gordon W.
Stuart A. Taylor
Robin C. Spiller
Penny A. Gowland
Luca Marciani Luca.Marciani@nottingham.ac.uk
Gordon W. Moran Gordon.Moran@nottingham.ac.uk
Background: Feeding triggers inter-related gastrointestinal (GI) motor, peptide and appetite responses. These are rarely studied together due to methodological limitations. Recent MRI advances allow pan-intestinal, non-invasive assessment of motility in the undisturbed gut.
This study aimed to develop a methodology to assess pan-intestinal motility and transit in a single session using MRI and compare imaging findings to GI peptide responses to a test meal and symptoms in a healthy volunteer cohort.
Methods: Fifteen healthy volunteers (29.3±2.7years and BMI 20.1±1.2Kg/m2) underwent baseline and postprandial MRI scans, symptom questionnaires and blood sampling (for subsequent GI peptide analysis, Glucagon-like peptide-1 (GLP-1), Polypeptide YY (PYY), Cholecystokinin (CCK)) at intervals for 270min following a 400g soup meal (204kcal, Heinz, UK). Gastric volume, gall bladder volume, small bowel water content, small bowel motility and whole gut transit were measured from the MRI scans.
Key Results: (mean±SEM) Small bowel motility index increased from fasting 39±3 arbitrary units (a.u.) to a maximum of 87±7a.u. immediately after feeding. PYY increased from fasting 98±10pg/ml to 149±14pg/ml at 30min and GLP-1 from fasting 15±3µg/ml to 22±4µg/ml. CCK increased from fasting 0.40±0.06pmol/ml to 0.94±0.1pmol/ml. Gastric volumes declined with a T1/2 of 46±5min and the gallbladder contracted from a fasting volume of 19±2ml to 12±2ml. Small bowel water content increased from 39±2ml to 51±2ml postprandial. Fullness VAS score increased from 9±5mm to 41±6mm at 30min postprandial.
Conclusions and Inferences: The test meal challenge was effective in inducing a change in MRI motility end-points which will improve understanding of the pathophysiological postprandial GI response.
|Journal Article Type||Article|
|Publication Date||Jan 1, 2018|
|Journal||Neurogastroenterology and Motility|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Khalaf, A., Hoad, C., Menys, A., Nowak, A., Taylor, S. A., Paparo, S., …Moran, G. W. (2018). MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans. Neurogastroenterology and Motility, 30(1), https://doi.org/10.1111/nmo.13182|
|Keywords||Gastrointestinal motility; Gut peptides; MRI; Satiety; Bowel|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf|
|Additional Information||This is the peer reviewed version of the following article: Khalaf A, Hoad CL, Menys A, et al. MRI assessment of the postprandial gastrointestinal motility and peptide response in healthy humans. Neurogastroenterol Motil. 2017;e13182. https://doi.org/10.1111/nmo.13182 which has been published in final form at http://onlinelibrary.wi.../10.1111/nmo.13182/full This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.|
MIC 1 COMPLETE.pdf
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
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