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The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science

Harding, Stephen E.; Wakefield, Jennifer; Gillis, Richard; Adams, Gary; McQueen, Caitlin

The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science Thumbnail


Authors

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STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry

Jennifer Wakefield

Richard Gillis

Caitlin McQueen



Abstract

The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a few nm to tens of microns, or in molecular weight, M (molar mass) terms from a few hundred Daltons to hundreds of millions. We illustrate this diversity by reviewing recent work on (i) small lignin-like isoeugenols of M ~0.4-0.9kDa for archaeological wood conservation (ii) protein-like association of a functional amino-cellulose, M=3.25kDa (iii) a small glycopeptide antibiotic (M~1.5 kDa) and its association with a protein involved in antibiotic resistance (M~47kDa) (iv) tetanus toxoid protein TTP (M~150kDa) and (v) the incorporation of TTP into two huge glycoconjugate vaccines of molecular weights reaching 100 MDa. In illustrating the diversity we will highlight developments in hydrodynamic analysis which have made the AUC such an exciting and important instrument, and point to a potential future development for extending its capability to highly concentrated systems.

Citation

Harding, S. E., Wakefield, J., Gillis, R., Adams, G., & McQueen, C. (2018). The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science. European Biophysics Journal, 47(7), 697–707. https://doi.org/10.1007/s00249-018-1290-6

Journal Article Type Article
Acceptance Date Jul 6, 2018
Online Publication Date Jul 28, 2018
Publication Date Oct 31, 2018
Deposit Date Jul 20, 2018
Publicly Available Date Aug 8, 2018
Journal European Biophysics Journal
Print ISSN 0175-7571
Electronic ISSN 1432-1017
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 47
Issue 7
Pages 697–707
DOI https://doi.org/10.1007/s00249-018-1290-6
Keywords Lignin; Amino-cellulose; Vancomycin; Tetanus toxoid; Glycovaccines
Public URL https://nottingham-repository.worktribe.com/output/945547
Publisher URL https://link.springer.com/article/10.1007%2Fs00249-018-1321-3
Contract Date Aug 8, 2018

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