Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia

Ercoli, Giuseppe; Fernandes, Vitor E.; Chung, Wen Y.; Wanford, Joseph J.; Thomson, Sarah; Bayliss, Christopher D.; Straatman, Kornelis; Crocker, Paul R.; Dennison, Ashley; Martinez-Pomares, Luisa; Andrew, Peter W.; Moxon, E. Richard; Oggioni, Marco R.

Abstract

Bacterial septicaemia is a major cause of mortality, but its pathogenesis remains poorly understood. In experimental pneumococcal murine intravenous infection, an initial reduction of bacteria in the blood is followed hours later by a fatal septicaemia. These events represent a population-bottleneck driven by efficient clearance of pneumococci by splenic macrophages and neutrophils, but as we show here, accompanied by occasional intracellular replication of bacteria that are taken up by a sub-set of CD169-positive splenic macrophages. In this model, proliferation of these sequestered bacteria provides a reservoir for dissemination of pneumococci into the bloodstream, as demonstrated by its prevention using an anti-CD169 mAb treatment. Intracellular replication of pneumococci within CD169+ plenic macrophages was also observed in an ex vivo porcine spleen, where the microanatomy is comparable to humans. We also showed that macrolides, that effectively penetrate macrophages, prevented septicaemia whereas beta-lactams, with inefficient intracellular penetration, failed to prevent dissemination to the blood. Our findings define a shift in our understanding of the pneumococcus from an exclusively extracellular pathogen to one with an intracellular phase. These findings open the door to the development of treatments that target this early, previously unrecognized intracellular phase of bacterial sepsis.

Citation

Ercoli, G., Fernandes, V. E., Chung, W. Y., Wanford, J. J., Thomson, S., Bayliss, C. D., Straatman, K., Crocker, P. R., Dennison, A., Martinez-Pomares, L., Andrew, P. W., Moxon, E. R., & Oggioni, M. R. (2018). Intracellular replication of Streptococcus pneumoniae inside splenic macrophages serves as a reservoir for septicaemia. Nature Microbiology, 3, https://doi.org/10.1038/s41564-018-0147-1

• Journal Article Type: Article
• Acceptance Date: Mar 8, 2018
• Online Publication Date: Apr 16, 2018
• Publication Date: Jun 1, 2018
• Deposit Date: Apr 17, 2018
• Publicly Available Date: Oct 17, 2018
• Journal: Nature Microbiology
• Electronic ISSN: 2058-5276
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 3
• DOI: https://doi.org/10.1038/s41564-018-0147-1
• Public URL: https://nottingham-repository.worktribe.com/output/935257
• Publisher URL: https://www.nature.com/articles/s41564-018-0147-1
• Contract Date: Apr 17, 2018