A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate

Giehl, Ricardo F. H.; Bhosale, Rahul; Giri, Jitender; Pandey, Bipin K.; Giehl, Ricardo F.H.; Hartmann, Anja; Traini, Richard; Truskina, Jekaterina; Leftley, Nicola; Hanlon, Meredith; Swarup, Kamal; Rashed, Afaf; Voß, Ute; Alonso, Jose; Stepanova, Anna; Yun, Jeonga; Ljung, Karin; Brown, Kathleen M.; Lynch, Jonathan P.; Dolan, Liam; Vernoux, Teva; Bishopp, Anthony; Wells, Darren; von Wirén, Nicolaus; Bennett, Malcolm J.; Swarup, Ranjan

Abstract

Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage for P in soil. Root hair elongation is particularly important since P is immobile. Here we report that auxin plays a critical role promoting root hair growth in Arabidopsis in response to low external P. Mutants disrupting auxin synthesis (taa1) and transport (aux1) attenuate the low P root hair response. Conversely, targeting AUX1 expression in lateral root cap and epidermal cells rescues this low P response in aux1. Hence auxin transport from the root apex to differentiation zone promotes auxin-dependent hair response to low P. Low external P results in induction of root hair expressed auxin-inducible transcription factors ARF19, RSL2, and RSL4. Mutants lacking these genes disrupt the low P root hair response. We conclude auxin synthesis, transport and response pathway components play critical roles regulating this low P root adaptive response.

Citation

Giehl, R. F. H., Bhosale, R., Giri, J., Pandey, B. K., Giehl, R. F., Hartmann, A., …Swarup, R. (2018). A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate. Nature Communications, 9(1), 1-9. https://doi.org/10.1038/s41467-018-03851-3

• Journal Article Type: Article
• Acceptance Date: Mar 16, 2018
• Online Publication Date: Apr 12, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Apr 12, 2018
• Publicly Available Date: Apr 12, 2018
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• Article Number: 1409
• Pages: 1-9
• DOI: https://doi.org/10.1038/s41467-018-03851-3
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Physics and Astronomy; General Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/924739
• Publisher URL: https://www.nature.com/articles/s41467-018-03851-3
• Additional Information: Received: 9 January 2017; Accepted: 16 March 2018; First Online: 12 April 2018; Change Date: 2 May 2018; Change Type: Correction; Change Details: The original version of this Article omitted the following from the Acknowledgements: ‘We also thank DBT-CREST BT/HRD/03/01/2002.’ This has been corrected in both the PDF and HTML versions of the Article.; : The authors declare no competing interests.
• Contract Date: Apr 12, 2018