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Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions

Xue, Xuan; Thiagarajan, Lalitha; Dixon, James E.; Saunders, Brian R; Shakesheff, Kevin M.; Alexander, Cameron


Xuan Xue

Lalitha Thiagarajan

Brian R Saunders

Kevin M. Shakesheff


Upper Critical Solution Temperature (UCST)-type thermally responsive polypeptides (TRPs) with phase transition temperatures around 37 ?C in phosphate-buffered saline (PBS) buffer (pH 7.4, 100 mM) were prepared from poly(L-ornithine) hydrobromide and coated on non-tissue culture-treated plastic plates (nTCP). Cell adhesion was observed at temperatures above the phase transition temperature of the coating polymer (39 ?C), while cell release was triggered when the culture temperature was switched to 37 ?C. Approximately 65% of the attached cells were released from the surface within 6 h after changing the temperature, and more than 96% of the released cells were viable. Water contact angle measurements performed at 39 and 37 ?C demonstrated that the surface hydrophobicity of the new TRP coatings changed in response to applied temperature. The cell attachment varied with the presence of serum in the media, suggesting that the TRP coatings mediated cell attachment and release as the underlying polymer surface changed conformation and consequently the display of adsorbed protein. These new TRP coatings provide an additional means to mediate cell attachment for application in cell-based tissue regeneration and therapies.


Xue, X., Thiagarajan, L., Dixon, J. E., Saunders, B. R., Shakesheff, K. M., & Alexander, C. (2018). Post-modified polypeptides with UCST-type behavior for control of cell attachment in physiological conditions. Materials, 11(1), Article 95.

Journal Article Type Article
Acceptance Date Jan 5, 2018
Publication Date Jan 9, 2018
Deposit Date Jan 30, 2018
Publicly Available Date Jan 30, 2018
Journal Materials
Electronic ISSN 1996-1944
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 11
Issue 1
Article Number 95
Keywords UCST polymer; thermo-responsive polypeptides; controlled cell attachment
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materials-11-00095-v2.pdf (3.7 Mb)

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