Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis
Chapman, Victoria; Markides, Hareklea; Sagar, Devi Rani; Xu, Luting; Burston, James J.; Mapp, Paul; Kay, Alasdair; Morris, Robert H.; Kehoe, Oksana; El Haj, Alicia J.
Devi Rani Sagar
James J. Burston
Robert H. Morris
Alicia J. El Haj
Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA.
Methods: Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5×106 late passage MSCs labelled with 10 μg/ml SiMAG, 1.5×106 late passage mesenchymal stem cells, the steroid Kenalog (200 μg/20 μL), 1.5×106 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint.
Results: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology.
Conclusion: Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.
|Journal Article Type||Article|
|Publication Date||Dec 24, 2017|
|Journal||Stem Cells International|
|Publisher||Hindawi Publishing Corporation|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Chapman, V., Markides, H., Sagar, D. R., Xu, L., Burston, J. J., Mapp, P., …El Haj, A. J. (2017). Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis. Stem Cells International, 2017, doi:10.1155/2017/2905104|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0|
Chapman et al_therapeutic benefit for late but not early.pdf
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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