Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis

Chapman, Victoria; Markides, Hareklea; Sagar, Devi Rani; Xu, Luting; Burston, James J.; Mapp, Paul; Kay, Alasdair; Morris, Robert H.; Kehoe, Oksana; El Haj, Alicia J.

Abstract

Background: Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA.
Methods: Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5×106 late passage MSCs labelled with 10 μg/ml SiMAG, 1.5×106 late passage mesenchymal stem cells, the steroid Kenalog (200 μg/20 μL), 1.5×106 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint.
Results: Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology.
Conclusion: Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.

Citation

Chapman, V., Markides, H., Sagar, D. R., Xu, L., Burston, J. J., Mapp, P., Kay, A., Morris, R. H., Kehoe, O., & El Haj, A. J. (2017). Therapeutic benefit for late, but not early, passage mesenchymal stem cells on pain behaviour in an animal model of osteoarthritis. Stem Cells International, 2017, https://doi.org/10.1155/2017/2905104

• Journal Article Type: Article
• Acceptance Date: Sep 7, 2017
• Publication Date: Dec 24, 2017
• Deposit Date: May 22, 2018
• Publicly Available Date: May 22, 2018
• Journal: Stem Cells International
• Print ISSN: 1687-966X
• Electronic ISSN: 1687-9678
• Publisher: Hindawi
• Peer Reviewed: Peer Reviewed
• Volume: 2017
• DOI: https://doi.org/10.1155/2017/2905104
• Public URL: https://nottingham-repository.worktribe.com/output/901678
• Publisher URL: https://www.hindawi.com/journals/sci/2017/2905104/
• Contract Date: May 22, 2018