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Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS

El-Hawiet, Amr; Chen, Yajie; Shams-Ud-Doha, Km; Kitova, Elena N.; Kitov, Pavel I.; Bode, Lars; Hage, Naim; Falcone, Franco H.; Klassen, John S.

Authors

Amr El-Hawiet

Yajie Chen

Km Shams-Ud-Doha

Elena N. Kitova

Pavel I. Kitov

Lars Bode

Naim Hage

Franco H. Falcone franco.falcone@nottingham.ac.uk

John S. Klassen



Abstract

© 2018 The Royal Society of Chemistry. Human milk oligosaccharides (HMOs) afford many health benefits to breast-fed infants, such as protection against infection and regulation of the immune system, through the formation of non-covalent interactions with protein receptors. However, the molecular details of these interactions are poorly understood. Here, we describe the application of catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) for screening natural libraries of HMOs against lectins. The HMOs in the libraries were first identified based on molecular weights (MWs), ion mobility separation arrival times (IMS-ATs) and collision-induced dissociation (CID) fingerprints of their deprotonated anions. The libraries were then screened against lectins and the ligands identified from the MWs, IMS-ATs and CID fingerprints of HMOs released from the lectin in the gas phase. To demonstrate the assay, four fractions, extracted from pooled human milk and containing ≥35 different HMOs, were screened against a C-terminal fragment of human galectin-3 (hGal-3C), for which the HMOs specificities have been previously investigated, and a fragment of the blood group antigen-binding adhesin (BabA) from Helicobacter pylori, for which the HMO specificities have not been previously established. The structures of twenty-one ligands, corresponding to both neutral and acidic HMOs, of hGal-3C were identified; all twenty-one were previously shown to be ligands for this lectin. The presence of HMO ligands at six other MWs was also ascertained. Application of the assay to BabA revealed nineteen specific HMO structures that are recognized by the protein and HMO ligands at two other MWs. Notably, it was found that BabA exhibits broad specificity for HMOs, and recognizes both neutral HMOs, including non-fucosylated ones, and acidic HMOs. The results of competitive binding experiments indicate that HMOs can interact with BabA at previously unknown binding sites. The affinities of eight purified HMOs for BabA were measured by ESI-MS and found to be in the 103 M-1 to 104 M-1 range.

Citation

El-Hawiet, A., Chen, Y., Shams-Ud-Doha, K., Kitova, E. N., Kitov, P. I., Bode, L., …Klassen, J. S. (2018). Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS. Analyst, 143(2), 536-548. https://doi.org/10.1039/c7an01397c

Journal Article Type Article
Acceptance Date Nov 27, 2017
Online Publication Date Nov 27, 2017
Publication Date Jan 21, 2018
Deposit Date Nov 30, 2017
Publicly Available Date Nov 28, 2018
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 143
Issue 2
Pages 536-548
DOI https://doi.org/10.1039/c7an01397c
Public URL http://eprints.nottingham.ac.uk/id/eprint/48408
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2017/AN/C7AN01397C#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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HMO paper accepted Analyst 2017.pdf (2.1 Mb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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