Tuneable 3D printed bioreactors for transaminations under continuous-flow

Peris, Edgar; Okafor, Obinna; Kulchinskaja, Evelina; Goodridge, Ruth D.; Luis, Santiago V.; Garcia-Verdugo, Eduardo; O'Reilly, Elaine; Sans, Victor

Abstract

A method to efficiently immobilize enzymes on 3D printed continuous-flow devices is presented. Application of these chemically modified devices enables rapid screening of immobilization mechanisms and reaction conditions, simple transfer of optimised conditions into tailored printed microfluidic reactors and development of continuous-flow biocatalytic processes. The bioreactors showed good activity (8-20.5 μmol h⁻¹ mgenz⁻¹) in the kinetic resolution of 1-methylbenzylamine, and very good stability (ca. 100 h under flow).

Citation

Peris, E., Okafor, O., Kulchinskaja, E., Goodridge, R. D., Luis, S. V., Garcia-Verdugo, E., O'Reilly, E., & Sans, V. (2017). Tuneable 3D printed bioreactors for transaminations under continuous-flow. Green Chemistry, 19(22), https://doi.org/10.1039/C7GC02421E

• Journal Article Type: Article
• Acceptance Date: Oct 3, 2017
• Online Publication Date: Oct 3, 2017
• Publication Date: Nov 21, 2017
• Deposit Date: Oct 4, 2017
• Publicly Available Date: Oct 4, 2018
• Journal: Green Chemistry
• Print ISSN: 1463-9262
• Electronic ISSN: 1463-9270
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 22
• DOI: https://doi.org/10.1039/C7GC02421E
• Public URL: https://nottingham-repository.worktribe.com/output/896447
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2017/GC/C7GC02421E#!divAbstract
• Contract Date: Oct 4, 2017