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Tuneable 3D printed bioreactors for transaminations under continuous-flow

Peris, Edgar; Okafor, Obinna; Kulchinskaja, Evelina; Goodridge, Ruth D.; Luis, Santiago V.; Garcia-Verdugo, Eduardo; O'Reilly, Elaine; Sans, Victor

Authors

Edgar Peris

Obinna Okafor Obinna.Okafor@nottingham.ac.uk

Evelina Kulchinskaja

RUTH GOODRIDGE Ruth.Goodridge@nottingham.ac.uk
Professor of Additive Manufacturing

Santiago V. Luis

Eduardo Garcia-Verdugo

Elaine O'Reilly

Victor Sans



Abstract

A method to efficiently immobilize enzymes on 3D printed continuous-flow devices is presented. Application of these chemically modified devices enables rapid screening of immobilization mechanisms and reaction conditions, simple transfer of optimised conditions into tailored printed microfluidic reactors and development of continuous-flow biocatalytic processes. The bioreactors showed good activity (8-20.5 μmol h⁻¹ mgenz⁻¹) in the kinetic resolution of 1-methylbenzylamine, and very good stability (ca. 100 h under flow).

Citation

Peris, E., Okafor, O., Kulchinskaja, E., Goodridge, R. D., Luis, S. V., Garcia-Verdugo, E., …Sans, V. (2017). Tuneable 3D printed bioreactors for transaminations under continuous-flow. Green Chemistry, 19(22), https://doi.org/10.1039/C7GC02421E

Journal Article Type Article
Acceptance Date Oct 3, 2017
Online Publication Date Oct 3, 2017
Publication Date Nov 21, 2017
Deposit Date Oct 4, 2017
Publicly Available Date Oct 4, 2018
Journal Green Chemistry
Print ISSN 1463-9262
Electronic ISSN 1463-9270
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 19
Issue 22
DOI https://doi.org/10.1039/C7GC02421E
Public URL http://eprints.nottingham.ac.uk/id/eprint/46977
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2017/GC/C7GC02421E#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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