Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery

Boitor, Radu; Kong, Kenny; Shipp, Dustin; Varma, Sandeep; Koloydenko, Alexey; Kusum, Kulkarni; Elsheikh, Somaia; Bakker Schut, Tom; Caspers, Peter; Puppels, Gerwin; Wolf, Martin van der; Sokolova, Elena; Nijsten, T.E.C.; Salence, Brogan; Williams, Hywel C.; Ioan, Notingher

Abstract

Multimodal spectral histopathology (MSH), an optical technique combining tissue auto-fluorescence (AF) imaging and Raman micro-spectroscopy (RMS), was previously proposed for detection of residual basal cell carcinoma (BCC) at the surface of surgically-resected skin tissue. Here we report the development of a fully-automated prototype instrument based on MSH designed to be used in the clinic and operated by a non-specialist spectroscopy user. The algorithms for the AF image processing and Raman spectroscopy classification had been first optimised on a manually-operated laboratory instrument and then validated on the automated prototype using skin samples from independent patients. We present results on a range of skin samples excised during Mohs micrographic surgery, and demonstrate consistent diagnosis obtained in repeat test measurement, in agreement with the reference histopathology diagnosis. We also show that the prototype instrument can be operated by clinical users (a skin surgeon and a core medical trainee, after only 1-8 hours of training) to obtain consistent results in agreement with histopathology. The development of the new automated prototype and demonstration of inter-instrument transferability of the diagnosis models are important steps on the clinical translation path: it allows the testing of the MSH technology in a relevant clinical environment in order to evaluate its performance on a sufficiently large number of patients.

Citation

Boitor, R., Kong, K., Shipp, D., Varma, S., Koloydenko, A., Kusum, K., …Ioan, N. (in press). Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery. Biomedical Optics Express, 8(12), https://doi.org/10.1364/BOE.8.005749

• Journal Article Type: Article
• Acceptance Date: Nov 13, 2017
• Online Publication Date: Nov 22, 2017
• Deposit Date: Nov 16, 2017
• Publicly Available Date: Nov 22, 2017
• Journal: Biomedical Optics Express
• Electronic ISSN: 2156-7085
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 12
• DOI: https://doi.org/10.1364/BOE.8.005749
• Public URL: https://nottingham-repository.worktribe.com/output/896140
• Publisher URL: https://www.osapublishing.org/boe/abstract.cfm?uri=boe-8-12-5749
• Contract Date: Nov 16, 2017