Oliver C Orji
Upregulated expression of FFAR2 and SOC3 genes is associated with gout
Orji, Oliver C; López-Domínguez, Maria B; Sandoval-Plata, Gabriela; Guetta-Baranes, Tamar; Valdes, Ana M; Doherty, Michael; Morgan, Kevin; Abhishek, Abhishek
Maria B López-Domínguez
Professor ANA VALDES Ana.Valdes@nottingham.ac.uk
Professor of Molecular & Genetic Epidemiology
KEVIN MORGAN firstname.lastname@example.org
Professor of Human Genomics and Molecular Genetics
ABHISHEK ABHISHEK ABHISHEK.ABHISHEK@NOTTINGHAM.AC.UK
Objective To examine the expression of Free fatty acid receptor 2 (FFAR2) and Suppressor of cytokine signalling 3 (SOCS3) genes in asymptomatic hyperuricaemia (AH), AH with MSU crystal deposition, inter-critical gout and gout flare.
Methods Study participants (n = 120) comprised 34 people with serum urate (SU) <360 μmol/l, 69 with AH ± MSU crystal deposition and 17 with a gout flare. Sixteen of the 17 patients with a gout flare attended a second visit 6–12 weeks later. Gene expression levels were assessed using RT-qPCR and results computed as fold changes (FC) after normalization to the reference gene.
Results FFAR2 was significantly upregulated during gout flares (FC = 2.9) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, P < 0.0001 for each comparison). FFAR2 was also significantly upregulated during inter-critical gout (FC = 1.8) compared with normal SU, AH and AH + MSU (FC = 1.1, P < 0.001 for each comparison). SOCS3 was significantly upregulated during gout flares (FC = 3.4) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, 1.1 and 1.2, respectively, P < 0.0001 for each comparison). SOCS3 was also upregulated during inter-critical gout (FC = 2.1) compared with normal SU (P = 0.02) and AH (P = 0.006) (FC = 1.1 and 1.2, respectively). FFAR2 expression was upregulated during gout flare compared with inter-critical gout and SOCS3 expression showed negative correlation with flare duration (r = –0.49, P < 0.05).
Conclusion FFAR2 upregulation is associated with gout and may trigger gout flares. SOCS3 may have a role in amelioration of gout flares.
Orji, O. C., López-Domínguez, M. B., Sandoval-Plata, G., Guetta-Baranes, T., Valdes, A. M., Doherty, M., …Abhishek, A. (2023). Upregulated expression of FFAR2 and SOC3 genes is associated with gout. Rheumatology, 62(2), 977-983. https://doi.org/10.1093/rheumatology/keac360
|Journal Article Type||Article|
|Acceptance Date||Jun 14, 2022|
|Online Publication Date||Jun 22, 2022|
|Deposit Date||Jul 7, 2022|
|Publicly Available Date||Jun 23, 2023|
|Publisher||Oxford University Press (OUP)|
|Peer Reviewed||Peer Reviewed|
|Keywords||Pharmacology (medical), Rheumatology|
Upregulated expression of FFAR2 and SOC3 genes is associated with Gout
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