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Upregulated expression of FFAR2 and SOC3 genes is associated with gout

Orji, Oliver C; López-Domínguez, Maria B; Sandoval-Plata, Gabriela; Guetta-Baranes, Tamar; Valdes, Ana M; Doherty, Michael; Morgan, Kevin; Abhishek, Abhishek

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Authors

Oliver C Orji

Maria B López-Domínguez

Gabriela Sandoval-Plata

Tamar Guetta-Baranes

Michael Doherty

KEVIN MORGAN kevin.morgan@nottingham.ac.uk
Professor of Human Genomics and Molecular Genetics



Abstract

Objective To examine the expression of Free fatty acid receptor 2 (FFAR2) and Suppressor of cytokine signalling 3 (SOCS3) genes in asymptomatic hyperuricaemia (AH), AH with MSU crystal deposition, inter-critical gout and gout flare.

Methods Study participants (n = 120) comprised 34 people with serum urate (SU) <360 μmol/l, 69 with AH ± MSU crystal deposition and 17 with a gout flare. Sixteen of the 17 patients with a gout flare attended a second visit 6–12 weeks later. Gene expression levels were assessed using RT-qPCR and results computed as fold changes (FC) after normalization to the reference gene.

Results FFAR2 was significantly upregulated during gout flares (FC = 2.9) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, P &amp;lt; 0.0001 for each comparison). FFAR2 was also significantly upregulated during inter-critical gout (FC = 1.8) compared with normal SU, AH and AH + MSU (FC = 1.1, P < 0.001 for each comparison). SOCS3 was significantly upregulated during gout flares (FC = 3.4) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, 1.1 and 1.2, respectively, P < 0.0001 for each comparison). SOCS3 was also upregulated during inter-critical gout (FC = 2.1) compared with normal SU (P = 0.02) and AH (P = 0.006) (FC = 1.1 and 1.2, respectively). FFAR2 expression was upregulated during gout flare compared with inter-critical gout and SOCS3 expression showed negative correlation with flare duration (r = –0.49, P < 0.05).

Conclusion FFAR2 upregulation is associated with gout and may trigger gout flares. SOCS3 may have a role in amelioration of gout flares.

Citation

Orji, O. C., López-Domínguez, M. B., Sandoval-Plata, G., Guetta-Baranes, T., Valdes, A. M., Doherty, M., …Abhishek, A. (2023). Upregulated expression of FFAR2 and SOC3 genes is associated with gout. Rheumatology, 62(2), 977-983. https://doi.org/10.1093/rheumatology/keac360

Journal Article Type Article
Acceptance Date Jun 14, 2022
Online Publication Date Jun 22, 2022
Publication Date 2023-02
Deposit Date Jul 7, 2022
Publicly Available Date Jun 23, 2023
Journal Rheumatology
Print ISSN 1462-0324
Electronic ISSN 1462-0332
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 62
Issue 2
Pages 977-983
DOI https://doi.org/10.1093/rheumatology/keac360
Keywords Pharmacology (medical), Rheumatology
Public URL https://nottingham-repository.worktribe.com/output/8853888
Publisher URL https://academic.oup.com/rheumatology/article/62/2/977/6613128

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