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Observations of extensive gene expression differences in the cerebellum and potential relevance to Alzheimer’s disease

Chappell, Sally; Patel, Tulsi; Guetta-Baranes, Tamar; Sang, Fei; Francis, Paul T.; Morgan, Kevin; Brookes, Keeley J.

Authors

Sally Chappell

Tulsi Patel

Tamar Guetta-Baranes

FEI SANG Fei.Sang@nottingham.ac.uk
Research Fellow

Paul T. Francis

KEVIN MORGAN kevin.morgan@nottingham.ac.uk
Professor of Human Genomics and Molecular Genetics

Keeley J. Brookes



Abstract

Objectives

In order to determine how gene expression is altered in disease it is of fundamental importance that the global distribution of gene expression levels across the disease-free brain are understood and how differences between tissue types might inform tissue choice for investigation of altered expression in disease state. The aim of this pilot project was to use RNA-sequencing to investigate gene expression differences between five general areas of post-mortem human brain (frontal, temporal, occipital, parietal and cerebellum), and in particular changes in gene expression in the cerebellum compared to cortex regions for genes relevant to Alzheimer’s disease, as the cerebellum is largely preserved from disease pathology and could be an area of interest for neuroprotective pathways.

Results

General gene expression profiles were found to be similar between cortical regions of the brain, however the cerebellum presented a distinct expression profile. Focused exploration of gene expression for genes associated with Alzheimer’s disease suggest that those involved in the immunity pathway show little expression in the brain. Furthermore some Alzheimer’s disease associated genes display significantly different expression in the cerebellum compared with other brain regions, which might indicate potential neuroprotective measures.

Journal Article Type Article
Publication Date Dec 1, 2018
Journal BMC Research Notes
Electronic ISSN 1756-0500
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 11
Issue 1
Article Number 646
APA6 Citation Chappell, S., Patel, T., Guetta-Baranes, T., Sang, F., Francis, P. T., Morgan, K., & Brookes, K. J. (2018). Observations of extensive gene expression differences in the cerebellum and potential relevance to Alzheimer’s disease. BMC Research Notes, 11(1), https://doi.org/10.1186/s13104-018-3732-8
DOI https://doi.org/10.1186/s13104-018-3732-8
Keywords RNA-sequence; Human brain; Cerebellum; Alzheimer’s disease
Publisher URL https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3732-8

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