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A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering

Boukari, Yamina; Qutachi, Omar; Morris, Andrew P.; Doughty, Stephen W.; Billa, Nashiru

Authors

Yamina Boukari yamina.boukari@gmail.com

Omar Qutachi omar.qutachi@nottingham.ac.uk

Andrew P. Morris andrew.morris@nottingham.edu.my

Stephen W. Doughty stephen.doughty@pmc.edu.my

Nashiru Billa Nashiru.Billa@nottingham.edu.my



Abstract

The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37°C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p < 0.05). The formulation also sintered at 37°C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37°C in situ.

Journal Article Type Article
Journal Journal of Biomaterials Science, Polymer Edition
Print ISSN 0920-5063
Electronic ISSN 0920-5063
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
APA6 Citation Boukari, Y., Qutachi, O., Morris, A. P., Doughty, S. W., & Billa, N. (in press). A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering. Journal of Biomaterials Science, Polymer Edition, doi:10.1080/09205063.2017.1364100
DOI https://doi.org/10.1080/09205063.2017.1364100
Keywords polymeric biomaterials, controlled delivery, poly (lactic-co-glycolic acid) (PLGA), microspheres, protein delivery, tissue engineering, mechanical properties, formulation
Publisher URL http://www.tandfonline.com/doi/full/10.1080/09205063.2017.1364100
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition on 17 August 2017, available online: http://www.tandfonline..../09205063.2017.1364100.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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