A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering

Boukari, Yamina; Qutachi, Omar; Morris, Andrew P.; Doughty, Stephen W.; Billa, Nashiru

Abstract

The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37°C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p < 0.05). The formulation also sintered at 37°C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37°C in situ.

Citation

Boukari, Y., Qutachi, O., Morris, A. P., Doughty, S. W., & Billa, N. (in press). A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering. Journal of Biomaterials Science, Polymer Edition, https://doi.org/10.1080/09205063.2017.1364100

• Journal Article Type: Article
• Acceptance Date: Aug 2, 2017
• Online Publication Date: Aug 17, 2017
• Deposit Date: Oct 11, 2017
• Publicly Available Date: Aug 18, 2018
• Journal: Journal of Biomaterials Science, Polymer Edition
• Print ISSN: 0920-5063
• Electronic ISSN: 1568-5624
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1080/09205063.2017.1364100
• Keywords: polymeric biomaterials, controlled delivery, poly (lactic-co-glycolic acid) (PLGA), microspheres, protein delivery, tissue engineering, mechanical properties, formulation
• Public URL: https://nottingham-repository.worktribe.com/output/878209
• Publisher URL: http://www.tandfonline.com/doi/full/10.1080/09205063.2017.1364100
• Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition on 17 August 2017, available online: http://www.tandfonline.com/10.1080/09205063.2017.1364100.
• Contract Date: Oct 11, 2017