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New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material

Chmayssem, Ayman; Tanase, Constantin Edi; Verplanck, Nicolas; Gougis, Maxime; Mourier, Véronique; Zebda, Abdelkader; Ghaemmaghami, Amir M.; Mailley, Pascal

New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material Thumbnail


Authors

Ayman Chmayssem

Constantin Edi Tanase

Nicolas Verplanck

Maxime Gougis

Véronique Mourier

Abdelkader Zebda

Pascal Mailley



Abstract

Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive monitoring of cell-cultures using EIS. In this paper, we reported the design and characterization of a modular EIS testing system based on a patented technology. This device was fabricated using easily processable methodologies including screen-printing of the impedance electrodes and molding or micromachining of the cell culture chamber with an easy assembly procedure. Accordingly, to obtain processable, biocompatible and sterilizable electrode materials that lower the impact of interfacial impedance on TEER (Transepithelial electrical resistance) measurements, and to enable concomitant microscopy observations, we optimized the formulation of the electrode inks and the design of the EIS electrodes, respectively. First, electrode materials were based on carbon biocompatible inks enriched with IrOx particles to obtain low interfacial impedance electrodes approaching the performances of classical non-biocompatible Ag/AgCl second-species electrodes. Secondly, we proposed three original electrode designs, which were compared to classical disk electrodes that were optically compatible with microscopy. We assessed the impact of the electrode design on the response of the impedance sensor using COMSOL Multiphysics. Finally, the performance of the impedance spectroscopy devices was assessed in vitro using human airway epithelial cell cultures.

Citation

Chmayssem, A., Tanase, C. E., Verplanck, N., Gougis, M., Mourier, V., Zebda, A., …Mailley, P. (2022). New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material. Biosensors, 12(7), Article 452. https://doi.org/10.3390/bios12070452

Journal Article Type Article
Acceptance Date Jun 21, 2022
Online Publication Date Jun 24, 2022
Publication Date Jul 1, 2022
Deposit Date Jul 26, 2022
Publicly Available Date Mar 28, 2024
Journal Biosensors
Electronic ISSN 2079-6374
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 12
Issue 7
Article Number 452
DOI https://doi.org/10.3390/bios12070452
Keywords Clinical Biochemistry; General Medicine; Analytical Chemistry; Biotechnology; Instrumentation; Biomedical Engineering; Engineering (miscellaneous)
Public URL https://nottingham-repository.worktribe.com/output/8771563
Publisher URL https://www.mdpi.com/2079-6374/12/7/452

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