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Hybrid bioactive hydroxyapatite/polycaprolactone nanoparticles for enhanced osteogenesis

El-Habashy, Salma E.; Eltaher, Hoda M.; Gaballah, Ahmed; Zaki, Eiman I.; Mehanna, Radwa A.; El-Kamel, Amal H.

Authors

Salma E. El-Habashy

Ahmed Gaballah

Eiman I. Zaki

Radwa A. Mehanna

Amal H. El-Kamel



Abstract

Hydroxyapatite nanoparticles (HApN) are largely employed as osteogenic inorganic material. Inorganic/polymeric hybrid nanostructures can provide versatile bioactivity for superior osteogenicity, particularly as nanoparticles. Herein, we present hybrid biomaterial-based hydroxyapatite/polycaprolactone nanoparticles (HAp/PCL NPs) realized using simple preparation techniques to augment HApN osteogenicity. Using wet chemical precipitation, we optimized HApN crystalline properties utilizing a 23-factorial design. Optimized HApN exhibited typical Ca/P elemental ratio with high reaction yield. Surface area analysis revealed their mesoporous nature and high surface area. Hybrid HAp/PCL NPs prepared using direct emulsification-solvent evaporation maintained HApN crystallinity with no observed chemical interactions. To the best of our knowledge, we are the first to elaborate the biocompatibility and osteogenicity of nanoparticulate hybrid HAp/PCL. Hybrid HAp/PCL NPs outperformed HApN regarding mesenchymal cell proliferation and osteodifferentiation with reduction of possible cytotoxicity. Unlike HApN, hybrid HAp/PCL NPs presented moderate expression of early osteogenic markers, Runx-2 and osteopontin and significantly elevated expression of the late osteogenic marker, bone sialoprotein after 10-day culture. Our results indicate that hybrid bioactive HAp/PCL NPs could offer a more prominent osteogenic potential than plain HApN for bone regenerative applications as a standalone nanoplatform or as part of complex engineered systems.

Citation

El-Habashy, S. E., Eltaher, H. M., Gaballah, A., Zaki, E. I., Mehanna, R. A., & El-Kamel, A. H. (2021). Hybrid bioactive hydroxyapatite/polycaprolactone nanoparticles for enhanced osteogenesis. Materials Science and Engineering: C, 119, Article 111599. https://doi.org/10.1016/j.msec.2020.111599

Journal Article Type Article
Acceptance Date Sep 23, 2020
Online Publication Date Oct 16, 2020
Publication Date 2021-02
Deposit Date Apr 26, 2023
Journal Materials Science and Engineering: C
Print ISSN 0928-4931
Electronic ISSN 1873-0191
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 119
Article Number 111599
DOI https://doi.org/10.1016/j.msec.2020.111599
Public URL https://nottingham-repository.worktribe.com/output/8771435
Publisher URL https://www.sciencedirect.com/science/article/pii/S0928493120335177?via%3Dihub