Nano zinc oxide-functionalized nanofibrous microspheres: A bioactive hybrid platform with antimicrobial, regenerative and hemostatic activities

Metwally, Walaa M.; El-Habashy, Salma E.; El-Nikhely, Nefertiti A.; Mahmoud, Hoda E.; Eltaher, Hoda M.; El-Khordagui, Labiba

Abstract

Bioactive hybrid constructs are at the cutting edge of innovative biomaterials. PLA nanofibrous microspheres (NF-MS) were functionalized with zinc oxide nanoparticles (nZnO) and DDAB-modified nZnO (D-nZnO) for developing inorganic/nano-microparticulate hybrid constructs (nZnO@NF-MS and D-nZnO@NF-MS) merging antibacterial, regenerative, and haemostatic functionalities. The hybrids appeared as three-dimensional NF-MS frameworks made-up entirely of interconnecting nanofibers embedding nZnO or D-nZnO. Both systems achieved faster release of Zn2+ than their respective nanoparticles and D-nZnO@NF-MS exhibited significantly greater surface wettability than nZnO@NF-MS. Regarding bioactivity, D-nZnO@NF-MS displayed a significantly greater and fast-killing effect against Staphylococcus aureus. Both nZnO@NF-MS and D-nZnO@NF-MS showed controllable concentration-dependent cytotoxicity to human gingival fibroblasts (HGF) compared with pristine NF-MS. They were also more effective than pristine NF-MS in promoting migration of human gingival fibroblasts (HGF) in the in vitro wound healing assay. Although D-nZnO@NF-MS showed greater in vitro hemostatic activity than nZnO@NF-MS (blood-clotting index 22.82 ± 0.65% vs.54.67 ± 2.32%), both structures exhibited instant hemostasis (0 s) with no blood loss (0 mg) in the rat-tail cutting technique. By merging the multiple therapeutic bioactivities of D-nZnO and the 3D-structural properties of NF-MS, the innovative D-nZnO@NF-MS hybrid construct provides a versatile bioactive material platform for different biomedical applications.

Citation

Metwally, W. M., El-Habashy, S. E., El-Nikhely, N. A., Mahmoud, H. E., Eltaher, H. M., & El-Khordagui, L. (2023). Nano zinc oxide-functionalized nanofibrous microspheres: A bioactive hybrid platform with antimicrobial, regenerative and hemostatic activities. International Journal of Pharmaceutics, 638, Article 122920. https://doi.org/10.1016/j.ijpharm.2023.122920

• Journal Article Type: Article
• Acceptance Date: Mar 31, 2023
• Online Publication Date: Apr 1, 2023
• Publication Date: May 10, 2023
• Deposit Date: Apr 26, 2023
• Journal: International Journal of Pharmaceutics
• Print ISSN: 0378-5173
• Electronic ISSN: 1873-3476
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 638
• Article Number: 122920
• DOI: https://doi.org/10.1016/j.ijpharm.2023.122920
• Keywords: Nanofibrous microspheres; Polylactic acid; Zinc oxide nanoparticles; DDAB; Cytotoxicity
• Public URL: https://nottingham-repository.worktribe.com/output/19294333
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S037851732300340X?via%3Dihub
• Additional Information: AAM requested 12 m embargo.