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Clinical impact of tumor DNA repair expression and T-cell infiltration in breast cancers

Green, Andrew R.; Aleskandarany, Mohammed A.; Ali, Reem; Hodgson, Eleanor Grace; Atabani, Suha; De Souza, Karen; Rakha, Emad; Ellis, Ian O.; Madhusudan, Srinivasan

Authors

Mohammed A. Aleskandarany

Reem Ali

Eleanor Grace Hodgson

Karen De Souza

EMAD RAKHA Emad.Rakha@nottingham.ac.uk
Professor of Breast Cancer Pathology



Abstract

© 2017 American Association for Cancer Research. Impaired DNA repair drives mutagenicity, which increases neoantigen load and immunogenicity. We investigated the expression of proteins involved in the DNA damage response (ATM, Chk2), double-strand break repair (BRCA1, BLM, WRN, RECQL4, RECQL5, TOPO2A, DNA-PKcs, Ku70/Ku80), nucleotide excision repair (ERCC1), base excision repair (XRCC1, pol β, FEN1, PARP1), and immune responses (CD8, PD-1, PD-L1, FOXP3) in 1,269 breast cancers and validated our findings in an independent estrogen receptor-negative (ER-) cohort (n = 279). Patients with tumors that expressed low XRCC1, low ATM, and low BRCA1 were not only associated with high numbers of CD8+ tumor-infiltrating lymphocytes, but were also linked to higher grades, high proliferation indexes, presence of dedifferentiated cells, ER- cells, and poor survival (all P < 0.01). PD-1+ or PD-L1+ breast cancers with low XRCC1 were also linked to an aggressive phenotype that was high grade, had high proliferation indexes, contained dedifferentiated cells and ER- (all with P values ≤ 0.01), and poor survival (P = 0.00021 and P = 0.00022, for PD-1+ and PD-L1+ cancers, respectively) including in an independent ER- validation cohort (P = 0.007 and P = 0.047, respectively). We conclude that the interplay between DNA repair, CD8, PD-L1, and PD-1 can promote aggressive tumor phenotypes. XRCC1-directed personalization of immune checkpoint inhibitor therapy may be feasible and warrants further investigation in breast cancer.

Citation

Green, A. R., Aleskandarany, M. A., Ali, R., Hodgson, E. G., Atabani, S., De Souza, K., …Madhusudan, S. (2017). Clinical impact of tumor DNA repair expression and T-cell infiltration in breast cancers. Cancer Immunology Research, 5(4), 292-299. https://doi.org/10.1158/2326-6066.CIR-16-0195

Journal Article Type Article
Acceptance Date Feb 20, 2017
Online Publication Date Mar 2, 2017
Publication Date 2017-04
Deposit Date May 24, 2017
Publicly Available Date May 24, 2017
Journal Cancer Immunology Research
Print ISSN 2326-6066
Electronic ISSN 2326-6074
Publisher American Association for Cancer Research
Peer Reviewed Peer Reviewed
Volume 5
Issue 4
Pages 292-299
DOI https://doi.org/10.1158/2326-6066.CIR-16-0195
Public URL http://eprints.nottingham.ac.uk/id/eprint/43070
Publisher URL http://cancerimmunolres.aacrjournals.org/content/5/4/292.long
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf







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