Skip to main content

Research Repository

Advanced Search

Mouse mitochondrial lipid composition is defined by age in brain and muscle


Amelia K. Pollard

Catharine A. Ortori


David A. Barrett


Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with high resolution mass spectrometry was used to establish and compare mitochondrial lipidomes of brain and skeletal muscle from young (4-11 weeks old) and middle age (78 weeks old) healthy mice. In middle age the brain mitochondria had reduced levels of fatty acids, particularly polyunsaturated fatty acids, while skeletal muscle mitochondria had a decreased abundance of phosphatidylethanolamine, but a pronounced increase of triglyceride levels. Reduced levels of phosphatidylethanolamines are known to decrease mitochondrial membrane fluidity and are connected with accelerated ageing. In mitochondria from skeletal muscle we propose that increased age causes a metabolic shift in the conversion of diacylglycerol so that triglycerides predominate compared with phosphatidylethanolamines. This is the first time mitochondrial lipid content in normal healthy mammalian ageing brain and muscle has been catalogued in detail across all lipid classes. We identify distinct mitochondrial lipid signatures that change with age, revealing tissue-specific lipid pathways as possible targets to ameliorate ageing-related mitochondrial decline.


Pollard, A. K., Ortori, C. A., Stöger, R., Barrett, D. A., & Chakrabarti, L. (in press). Mouse mitochondrial lipid composition is defined by age in brain and muscle. Aging, 9(3),

Journal Article Type Article
Acceptance Date Mar 13, 2017
Online Publication Date Mar 21, 2017
Deposit Date Mar 15, 2017
Publicly Available Date Mar 21, 2017
Journal Aging
Electronic ISSN 1945-4589
Publisher Impact Journals
Peer Reviewed Peer Reviewed
Volume 9
Issue 3
Keywords mitochondria, global lipidomics, ageing, mouse, skeletal muscle, brain
Public URL
Publisher URL


Y4X9ErNC27mq83gFb.pdf (2.2 Mb)

Copyright Statement
Copyright information regarding this work can be found at the following address:

You might also like

Downloadable Citations