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Quantification and qualitative effects of different PEGylations on Poly(butyl cyanoacrylate) nanoparticles


Andreas K.O Aslund

Einar Sulheim

Sofie Snipstad

Eva von Haartman

Habib Baghirov


Principal Research Fellow

de Lange Davies Catharina

Schmid Ruth


Protein adsorption on nanoparticles (NPs) used in nanomedicine leads to opsonization and activation of the complement system in blood, which substantially reduces the blood circulation time of NPs. The most commonly used method to avoid protein adsorption, is to coat the NPs with polyethylene glycol, so called PEGylation. Although PEGylation is of utmost importance for designing the in vivo behavior of the NP, there is still a considerable lack of methods for characterization and fundamental understanding related to the PEGylation of NPs. In this work we have studied four different poly(butyl cyanoacrylate) (PBCA) NPs , PEGylated with different types of PEG-based non-ionic surfactants–Jeffamine M-2070, Brij L23, Kolliphor HS 15, Pluronic F68–or combinations thereof. We evaluated the PEGylation, both quantitatively by nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), and qualitatively by studying zeta-potential, protein adsorption, diffusion, cellular interactions and blood circulation half-life. We found that NMR and ToF-SIMS are complementary methods, while TGA is less suitable to quantitate PEG on polymeric NPs. It was found that longer PEG increases both blood circulation time and diffusion of NPs in collagen gels.


Aslund, A. K., Sulheim, E., Snipstad, S., von Haartman, E., Baghirov, H., Starr, N. J., …Ýrr, M. (in press). Quantification and qualitative effects of different PEGylations on Poly(butyl cyanoacrylate) nanoparticles. Molecular Pharmaceutics, 14(8),

Journal Article Type Article
Acceptance Date Feb 7, 2017
Online Publication Date Feb 7, 2017
Deposit Date Apr 10, 2017
Publicly Available Date Apr 10, 2017
Journal Molecular Pharmaceutics
Print ISSN 1543-8384
Electronic ISSN 1543-8384
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 14
Issue 8
Keywords PACA, PEG, NMR, ToF-SIMS, TGA, circulation time, protein adsorption
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