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Allergenicity and oral tolerance of enzymatic cross-linked tropomyosin evaluated using cell and mouse models

Liu, Guang-Yu; Hu, Meng-Jun; Sun, Le-Chang; Han, Xin-Yu; Liu, Qing-Mei; Alcocer, Marcos J.C.; Fei, Dan-Xia; Cao, Min-Jie; Liu, Guang-Ming

Authors

Guang-Yu Liu

Meng-Jun Hu

Le-Chang Sun

Xin-Yu Han

Qing-Mei Liu

Marcos J.C. Alcocer

Dan-Xia Fei

Min-Jie Cao

Guang-Ming Liu



Abstract

The enzymatic cross-linking of proteins to form high-molecular-weight compounds may alter their sensitization potential. The IgG-/IgE-binding activity, digestibility, allergenicity, and oral tolerance of cross-linked tropomyosin with tyrosinase (CTC) or horseradish peroxidase (CHP) were investigated. ELISA results demonstrated CTC or CHP reduced its IgE-binding activity by 34.5 ± 1.8 and 63.5 ± 0.6%, respectively. Compared with native tropomyosin or CTC, CHP was more easily digested into small fragments; CHP decreased the degranulation of RBL-2H3 cells and increased endocytosis by dendritic cells. CHP can induce oral tolerance and reduce allergenicity in mice by decreasing IgE and IgG1 levels in serum, the production of T-cell cytokines, and the percentage composition of dendritic cells. These findings demonstrate CHP has more potential of reducing the allergenicity than CTC via influencing the morphology of protein, changing the original method of antigen presentation, modulating the Th1/Th2 immunobalance, and inducing the oral tolerance of the allergen tropomyosin.

Journal Article Type Article
Journal Journal of Agricultral and Food Chemistry
Print ISSN 0021-8561
Electronic ISSN 1520-5118
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 65
Issue 10
APA6 Citation Liu, G., Hu, M., Sun, L., Han, X., Liu, Q., Alcocer, M. J., …Liu, G. (in press). Allergenicity and oral tolerance of enzymatic cross-linked tropomyosin evaluated using cell and mouse models. Journal of Agricultural and Food Chemistry, 65(10), doi:10.1021/acs.jafc.6b05816
DOI https://doi.org/10.1021/acs.jafc.6b05816
Keywords allergenicity reducing; enzymatic cross-linking; horseradish peroxidase; oral tolerance potency; tropomyosin
Publisher URL http://pubs.acs.org/doi/abs/10.1021/acs.jafc.6b05816
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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