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Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Bilverstone, T.W.; Kinsmore, N.L.; Minton, Nigel P.; Kuehne, Sarah A.

Authors

T.W. Bilverstone

N.L. Kinsmore

Nigel P. Minton nigel.minton@nottingham.ac.uk

Sarah A. Kuehne



Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Journal Article Type Article
Journal Anaerobe
Print ISSN 1075-9964
Electronic ISSN 1095-8274
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 44
APA6 Citation Bilverstone, T., Kinsmore, N., Minton, N. P., & Kuehne, S. A. (in press). Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44, https://doi.org/10.1016/j.anaerobe.2017.01.009
DOI https://doi.org/10.1016/j.anaerobe.2017.01.009
Keywords C. difficile; PaLoc; Binary toxin; CdtR; Virulence
Publisher URL http://www.sciencedirect.com/science/article/pii/S1075996417300173
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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