Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels
Bilverstone, T.W.; Kinsmore, N.L.; Minton, Nigel P.; Kuehne, Sarah A.
Nigel P. Minton email@example.com
Sarah A. Kuehne
Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.
|Journal Article Type||Article|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Bilverstone, T., Kinsmore, N., Minton, N. P., & Kuehne, S. A. (in press). Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44, https://doi.org/10.1016/j.anaerobe.2017.01.009|
|Keywords||C. difficile; PaLoc; Binary toxin; CdtR; Virulence|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0|
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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