@article { , title = {Development of Clostridium difficile R20291?PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels}, abstract = {Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291?PaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.}, doi = {10.1016/j.anaerobe.2017.01.009}, eissn = {1095-8274}, issn = {1075-9964}, journal = {Anaerobe}, publicationstatus = {Published}, publisher = {Elsevier}, url = {https://nottingham-repository.worktribe.com/output/840051}, volume = {44}, keyword = {C. difficile, PaLoc, Binary toxin, CdtR, Virulence}, author = {Bilverstone, T.W. and Kinsmore, N.L. and Minton, Nigel P. and Kuehne, Sarah A.} }