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Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Bilverstone, T.W.; Kinsmore, N.L.; Minton, Nigel P.; Kuehne, Sarah A.

Authors

T.W. Bilverstone

N.L. Kinsmore

NIGEL MINTON nigel.minton@nottingham.ac.uk
Professor of Appliedmolecular Microbiology

Sarah A. Kuehne



Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Citation

Bilverstone, T., Kinsmore, N., Minton, N. P., & Kuehne, S. A. (in press). Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44, https://doi.org/10.1016/j.anaerobe.2017.01.009

Journal Article Type Article
Acceptance Date Jan 14, 2017
Online Publication Date Jan 17, 2017
Deposit Date Jan 23, 2017
Publicly Available Date Jan 23, 2017
Journal Anaerobe
Print ISSN 1075-9964
Electronic ISSN 1095-8274
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 44
DOI https://doi.org/10.1016/j.anaerobe.2017.01.009
Keywords C. difficile; PaLoc; Binary toxin; CdtR; Virulence
Public URL http://eprints.nottingham.ac.uk/id/eprint/39999
Publisher URL http://www.sciencedirect.com/science/article/pii/S1075996417300173
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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