Development of Clostridium difficile R20291?PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Bilverstone, T.W.; Kinsmore, N.L.; Minton, Nigel P.; Kuehne, Sarah A.

Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291?PaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Citation

Bilverstone, T., Kinsmore, N., Minton, N. P., & Kuehne, S. A. (in press). Development of Clostridium difficile R20291?PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels. Anaerobe, 44, https://doi.org/10.1016/j.anaerobe.2017.01.009

• Journal Article Type: Article
• Acceptance Date: Jan 14, 2017
• Online Publication Date: Jan 17, 2017
• Deposit Date: Jan 23, 2017
• Publicly Available Date: Jan 23, 2017
• Journal: Anaerobe
• Print ISSN: 1075-9964
• Electronic ISSN: 1095-8274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 44
• DOI: https://doi.org/10.1016/j.anaerobe.2017.01.009
• Keywords: C. difficile; PaLoc; Binary toxin; CdtR; Virulence
• Public URL: https://nottingham-repository.worktribe.com/output/840051
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1075996417300173