Skip to main content

Research Repository

Advanced Search

Intra-arterial nitroglycerin as directed acute treatment in experimental ischemic stroke

Maniskas, Michael E.; Roberts, Jill M.; Trueman, Rebecca C.; Learoyd, Annastazia E.; Gorman, Amanda; Fraser, Justin F.; Bix, Gregory J.

Intra-arterial nitroglycerin as directed acute treatment in experimental ischemic stroke Thumbnail


Authors

Michael E. Maniskas

Jill M. Roberts

Rebecca C. Trueman

Annastazia E. Learoyd

Amanda Gorman

Justin F. Fraser

Gregory J. Bix



Abstract

Background: Nitroglycerin (also known as glyceryl trinitrate (GTN)), a vasodilator best known for treatment of ischemic heart disease, has also been investigated for its potential therapeutic benefit in ischemic stroke. The completed Efficacy of Nitric Oxide in Stroke trial suggested that GTN has therapeutic benefit with acute (within 6 hours) transdermal systemic sustained release therapy.
Objective: To examine an alternative use of GTN as an acute therapy for ischemic stroke following successful recanalization.
Methods: We administered GTN IA following transient middle cerebral artery occlusion in mice. Because no standard dose of GTN is available following emergent large vessel occlusion, we performed a dose–response (3.12, 6.25, 12.5, and 25 .ig/.iL) analysis. Next, we looked at blood perfusion (flow) through the middle cerebral artery using laser Doppler flowmetry. Functional outcomes, including forced motor movement rotor rod, were assessed in the 3.12, 6.25, and 12.5 .ig/.iL groups. Histological analysis was performed using cresyl violet for infarct volume, and glial fibrillary activating protein (GFAP) and NeuN immunohistochemistry for astrocyte activation and mature neuron survival, respectively.
Results: Overall, we found that acute post-stroke IA GTN had little effect on vessel dilatation after 15 min. Functional analysis showed a significant difference between GTN (3.12 and 6.25 .ig/.iL) and control at post-stroke day 1. Histological measures showed a significant reduction in infarct volume and GFAP immunoreactivity and a significant increase in NeuN.
Conclusions: These results demonstrate that acute IA GTN is neuroprotective in experimental ischemic stroke and warrants further study as a potentially new stroke therapy.

Citation

Maniskas, M. E., Roberts, J. M., Trueman, R. C., Learoyd, A. E., Gorman, A., Fraser, J. F., & Bix, G. J. (in press). Intra-arterial nitroglycerin as directed acute treatment in experimental ischemic stroke. Journal of NeuroInterventional Surgery, 10(1), https://doi.org/10.1136/neurintsurg-2016-012793

Journal Article Type Article
Acceptance Date Dec 10, 2016
Online Publication Date Dec 28, 2016
Deposit Date Feb 15, 2017
Publicly Available Date Mar 28, 2024
Journal Journal of NeuroInterventional Surgerey
Print ISSN 1759-8478
Electronic ISSN 1759-8486
Publisher BMJ Publishing Group
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
DOI https://doi.org/10.1136/neurintsurg-2016-012793
Public URL https://nottingham-repository.worktribe.com/output/831993
Publisher URL http://jnis.bmj.com/content/early/2016/12/28/neurintsurg-2016-012793

Files





You might also like



Downloadable Citations