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A genetic assay for gene essentiality in Clostridium

Walker, David J.F.; Heap, John T.; Winzer, Klaus; Minton, Nigel P.

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Authors

David J.F. Walker

John T. Heap



Abstract

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Citation

Walker, D. J., Heap, J. T., Winzer, K., & Minton, N. P. (2016). A genetic assay for gene essentiality in Clostridium. Anaerobe, 42, 40-43. https://doi.org/10.1016/j.anaerobe.2016.07.007

Journal Article Type Article
Acceptance Date Jul 29, 2016
Online Publication Date Jul 31, 2016
Publication Date 2016-12
Deposit Date Aug 22, 2016
Publicly Available Date Mar 28, 2024
Journal Anaerobe
Print ISSN 1075-9964
Electronic ISSN 1095-8274
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 42
Pages 40-43
DOI https://doi.org/10.1016/j.anaerobe.2016.07.007
Keywords Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target
Public URL https://nottingham-repository.worktribe.com/output/831627
Publisher URL http://www.sciencedirect.com/science/article/pii/S1075996416300919
Additional Information This article is maintained by: Elsevier; Article Title: A genetic assay for gene essentiality in Clostridium; Journal Title: Anaerobe; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.anaerobe.2016.07.007; Content Type: article; Copyright: © 2016 The Authors. Published by Elsevier Ltd.

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