A genetic assay for gene essentiality in Clostridium

Walker, David J.F.; Heap, John T.; Winzer, Klaus; Minton, Nigel P.

Abstract

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Citation

Walker, D. J., Heap, J. T., Winzer, K., & Minton, N. P. (2016). A genetic assay for gene essentiality in Clostridium. Anaerobe, 42, 40-43. https://doi.org/10.1016/j.anaerobe.2016.07.007

• Journal Article Type: Article
• Acceptance Date: Jul 29, 2016
• Online Publication Date: Jul 31, 2016
• Publication Date: 2016-12
• Deposit Date: Aug 22, 2016
• Publicly Available Date: Aug 22, 2016
• Journal: Anaerobe
• Print ISSN: 1075-9964
• Electronic ISSN: 1095-8274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 42
• Pages: 40-43
• DOI: https://doi.org/10.1016/j.anaerobe.2016.07.007
• Keywords: Clostridium difficile; ClosTron; merodiploid; gene essentiality; therapeutic target
• Public URL: https://nottingham-repository.worktribe.com/output/831627
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1075996416300919
• Additional Information: This article is maintained by: Elsevier; Article Title: A genetic assay for gene essentiality in Clostridium; Journal Title: Anaerobe; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.anaerobe.2016.07.007; Content Type: article; Copyright: © 2016 The Authors. Published by Elsevier Ltd.
• Contract Date: Aug 22, 2016