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Extracellular matrix-derived hydrogels for dental stem cell delivery

Viswanath, Aiswarya; Vanacker, Julie; Germain, Loic; Leprince, Julien G.; Diogenes, Anibal; Shakesheff, Kevin M.; White, Lisa J.; des Rieux, Anne

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Aiswarya Viswanath

Julie Vanacker

Loic Germain

Julien G. Leprince

Anibal Diogenes

Kevin M. Shakesheff

Anne des Rieux


Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord and dentine ECM hydrogels exhibited distinct structural, mechanical and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behaviour for constructive spinal cord regeneration

Journal Article Type Article
Acceptance Date Sep 13, 2016
Publication Date Nov 5, 2016
Deposit Date Sep 15, 2017
Publicly Available Date Sep 15, 2017
Journal Journal of Biomedical Materials Research Part A
Print ISSN 1549-3296
Electronic ISSN 1552-4965
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 105
Issue 1
Pages 319-328
Keywords ECM hydrogels, Dental stem cell delivery, Spinal cord, Bone, Dentine
Public URL
Publisher URL;jsessionid=F19EBF527F73CE2DCC30A5CE2328E855.f02t01
Additional Information This is the peer reviewed version of the following article: Viswanath A, Vanacker J, Germain L, Leprince JG, Diogenes A, Shakesheff KM, White LJ, des Rieux A. 2017. Extracellular matrix-derived hydrogels for dental stem cell delivery. J Biomed Mater Res Part A 2017:105A:319–328, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.


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