Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro

Woodham, Andrew W.; Sanna, Adriana M.; Taylor, Julia R.; Skeate, Joseph G.; Da Silva, Diane M.; Dekker, Lodewijk V.; Kast, W. Martin

Abstract

© 2016 The Author(s). During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique. Additionally, inhibitors of A2t (A2ti) that target the interaction between A2 and S100A10 were tested for their ability to impair productive HIV-1 infection of macrophages. Our data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. Furthermore, an anti-A2 antibody impaired HIV-1 particle production in macrophages in vitro, whereas A2ti did not indicating that annexin A2 may promote HIV-1 infection of macrophages in its monomeric rather than tetrameric form.

Citation

Woodham, A. W., Sanna, A. M., Taylor, J. R., Skeate, J. G., Da Silva, D. M., Dekker, L. V., & Kast, W. M. (2016). Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro. Virology Journal, 13(1), 1-5. https://doi.org/10.1186/s12985-016-0649-5

• Journal Article Type: Article
• Acceptance Date: Nov 10, 2016
• Online Publication Date: Nov 18, 2016
• Publication Date: Nov 18, 2016
• Deposit Date: Feb 27, 2017
• Publicly Available Date: Feb 27, 2017
• Journal: Virology Journal
• Electronic ISSN: 1743-422X
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 1
• Article Number: 187
• Pages: 1-5
• DOI: https://doi.org/10.1186/s12985-016-0649-5
• Keywords: Annexin A2, Annexin A2, heterotetramer, HIV-1, Inhibitor, Macrophage, Receptor
• Public URL: https://nottingham-repository.worktribe.com/output/828024
• Publisher URL: https://virologyj.biomedcentral.com/articles/10.1186/s12985-016-0649-5
• Contract Date: Feb 27, 2017