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QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems

Zhang, Ying; Thomas, Catherine L.; Xiang, Jinxia; Long, Yan; Wang, Xiaohua; Zou, Jun; Luo, Ziliang; Ding, Guangda; Cai, Hongmei; Graham, Neil S.; Hammond, John P.; King, Graham J.; White, Philip J.; Xu, Fangsen; Broadley, Martin R.; Shi, Lei; Meng, Jinling

Authors

YING ZHANG YING.ZHANG@NOTTINGHAM.AC.UK
Senior Research Fellow

Catherine L. Thomas

Jinxia Xiang

Yan Long

Xiaohua Wang

Jun Zou

Ziliang Luo

Guangda Ding

Hongmei Cai

NEIL GRAHAM neil.graham@nottingham.ac.uk
Senior Research Fellow

John P. Hammond

Graham J. King

Philip J. White

Fangsen Xu

Lei Shi

Jinling Meng



Abstract

© The Author(s) 2016. A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average marker density which increased from 0.39 to 0.97 (0.82 SNP bin) per cM. Root and shoot traits were screened under low and 'normal' phosphate (Pi) supply using a 'pouch and wick' system, and had been screened previously in an agar based system. The P-efficient parent Ningyou7 had a shorter primary root length (PRL), greater lateral root density (LRD) and a greater shoot biomass than the P-inefficient parent Tapidor under both treatments and growth systems. Quantitative trait loci (QTL) analysis identified a total of 131 QTL, and QTL meta-analysis found four integrated QTL across the growth systems. Integration reduced the confidence interval by ∼41%. QTL for root and shoot biomass were co-located on chromosome A3 and for lateral root emergence were co-located on chromosomes A4/C4 and C8/C9. There was a major QTL for LRD on chromosome C9 explaining ∼18% of the phenotypic variation. QTL underlying an increased LRD may be a useful breeding target for P uptake efficiency in Brassica.

Citation

Zhang, Y., Thomas, C. L., Xiang, J., Long, Y., Wang, X., Zou, J., …Meng, J. (2016). QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems. Scientific Reports, 6(1), https://doi.org/10.1038/srep33113

Journal Article Type Article
Acceptance Date Aug 22, 2016
Online Publication Date Sep 14, 2016
Publication Date Sep 14, 2016
Deposit Date Nov 7, 2016
Publicly Available Date Nov 7, 2016
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 6
Issue 1
Article Number 33113
DOI https://doi.org/10.1038/srep33113
Public URL http://eprints.nottingham.ac.uk/id/eprint/38556
Publisher URL http://dx.doi.org/10.1038/srep33113
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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