Thomas D. Alcock
Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa
Alcock, Thomas D.; Thomas, Catherine L.; � Lochlainn, Seosamh; Pongrac, Paula; Wilson, Michael; Moore, Christopher; Reyt, Guilhem; Vogel-Miku�, Katarina; Kelemen, Mitja; Hayden, Rory; Wilson, Lolita; Stephenson, Pauline; �stergaard, Lars; Irwin, Judith A.; Hammond, John P.; King, Graham J.; Salt, David E.; Graham, Neil S.; White, Philip J.; Broadley, Martin R.
Authors
Catherine L. Thomas
Seosamh � Lochlainn
Paula Pongrac
MICHAEL WILSON MICHAEL.WILSON@NOTTINGHAM.AC.UK
Senior Technical Specialist
Christopher Moore
Guilhem Reyt
Katarina Vogel-Miku�
Mitja Kelemen
Rory Hayden
Lolita Wilson
Pauline Stephenson
Lars �stergaard
Judith A. Irwin
John P. Hammond
Graham J. King
David E. Salt
NEIL GRAHAM NEIL.GRAHAM@NOTTINGHAM.AC.UK
Senior Research Fellow
Philip J. White
MARTIN BROADLEY MARTIN.BROADLEY@NOTTINGHAM.AC.UK
Professor of Plant Nutrition
Abstract
Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thaliana). Analysis of the spatial distribution of elements showed elevated extracellular Mg concentrations in leaves of braA.sgn3.a-1, hypothesized to result from preferential export of excessive Mg from cells to ensure suitable cellular concentrations. This work confirms a conserved role of SGN3 in controlling nutrient homeostasis in B. rapa, and reveals mechanisms by which plants are able to deal with perturbed shoot element concentrations resulting from a “leaky” root endodermal barrier. Characterization of variation in leaf Mg and Ca accumulation across a mutagenized population of B. rapa shows promise for using such populations in breeding programs to increase edible concentrations of essential human and animal nutrients.
Citation
Alcock, T. D., Thomas, C. L., Ó Lochlainn, S., Pongrac, P., Wilson, M., Moore, C., …Broadley, M. R. (2021). Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa. Plant Physiology, 186(3), 1616-1631. https://doi.org/10.1093/PLPHYS/KIAB150
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 12, 2021 |
Online Publication Date | Apr 8, 2021 |
Publication Date | Jul 1, 2021 |
Deposit Date | Apr 27, 2021 |
Publicly Available Date | Apr 27, 2021 |
Journal | Plant Physiology |
Print ISSN | 0032-0889 |
Electronic ISSN | 1532-2548 |
Publisher | American Society of Plant Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 186 |
Issue | 3 |
Pages | 1616-1631 |
DOI | https://doi.org/10.1093/PLPHYS/KIAB150 |
Keywords | Plant Science; Genetics; Physiology |
Public URL | https://nottingham-repository.worktribe.com/output/5469720 |
Publisher URL | https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiab150/6217901 |
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Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa
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Publisher Licence URL
https://creativecommons.org/licenses/by-nc-nd/4.0/
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