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Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals

Naqid, Ibrahim A.; Owen, Jonathan P.; Maddison, Ben C.; Spiliotopoulos, Anastasios; Emes, Richard D.; Warry, Andrew; Flynn, Robin J.; Martelli, Francesca; Gosling, Rebecca J.; Davies, Robert H.; La Ragione, Roberto M.; Gough, Kevin C.

Authors

Ibrahim A. Naqid

Jonathan P. Owen

Ben C. Maddison

Anastasios Spiliotopoulos

RICHARD EMES richard.emes@nottingham.ac.uk
Professor of Bioinformatics

Andrew Warry

Robin J. Flynn

Francesca Martelli

Rebecca J. Gosling

Robert H. Davies

Roberto M. La Ragione

KEVIN GOUGH kevin.gough@nottingham.ac.uk
Professor of Biochemistry and Pathology



Abstract

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from vaccinated animals (DIVA tests) for established killed or attenuated vaccines. Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis to identify peptides that were preferentially bound by IgY from chickens infected with Salmonella Typhimurium or S. Enteritidis compared to IgY from vaccinates, for both an attenuated and an inactivated commercial vaccine. Peptides that were highly enriched against IgY from at least 4 out of 10 infected chickens were selected: 18 and 12 peptides for the killed and attenuated vaccines, respectively. The ten most discriminatory peptides for each vaccine were identi ed in an ELISA using a training set of IgY samples. These peptides were then used in multi-peptide assays that, when analysing a wider set of samples from infected and vaccinated animals, diagnosed infection with 100% sensitivity and speci city. The data describes a method for the development of DIVA assays for conventional attenuated and killed vaccines.

Citation

Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., …Gough, K. C. (2016). Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, https://doi.org/10.1038/srep31186

Journal Article Type Article
Acceptance Date Jul 13, 2016
Online Publication Date Aug 11, 2016
Publication Date Aug 11, 2016
Deposit Date Nov 7, 2016
Publicly Available Date Nov 7, 2016
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 6
Article Number 31186
DOI https://doi.org/10.1038/srep31186
Public URL http://eprints.nottingham.ac.uk/id/eprint/38514
Publisher URL http://www.nature.com/articles/srep31186
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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