Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals

Naqid, Ibrahim A.; Owen, Jonathan P.; Maddison, Ben C.; Spiliotopoulos, Anastasios; Emes, Richard D.; Warry, Andrew; Flynn, Robin J.; Martelli, Francesca; Gosling, Rebecca J.; Davies, Robert H.; La Ragione, Roberto M.; Gough, Kevin C.

Abstract

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from vaccinated animals (DIVA tests) for established killed or attenuated vaccines. Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis to identify peptides that were preferentially bound by IgY from chickens infected with Salmonella Typhimurium or S. Enteritidis compared to IgY from vaccinates, for both an attenuated and an inactivated commercial vaccine. Peptides that were highly enriched against IgY from at least 4 out of 10 infected chickens were selected: 18 and 12 peptides for the killed and attenuated vaccines, respectively. The ten most discriminatory peptides for each vaccine were identi ed in an ELISA using a training set of IgY samples. These peptides were then used in multi-peptide assays that, when analysing a wider set of samples from infected and vaccinated animals, diagnosed infection with 100% sensitivity and speci city. The data describes a method for the development of DIVA assays for conventional attenuated and killed vaccines.

Citation

Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., Flynn, R. J., Martelli, F., Gosling, R. J., Davies, R. H., La Ragione, R. M., & Gough, K. C. (2016). Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, Article 31186. https://doi.org/10.1038/srep31186

• Journal Article Type: Article
• Acceptance Date: Jul 13, 2016
• Online Publication Date: Aug 11, 2016
• Publication Date: Aug 11, 2016
• Deposit Date: Nov 7, 2016
• Publicly Available Date: Nov 7, 2016
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Article Number: 31186
• DOI: https://doi.org/10.1038/srep31186
• Public URL: https://nottingham-repository.worktribe.com/output/805878
• Publisher URL: http://www.nature.com/articles/srep31186
• Contract Date: Nov 7, 2016