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Dynamic regulation of auxin oxidase and conjugating enzymes AtDAO1 and GH3 modulates auxin homeostasis


Associate Professor

Ondrej Novak

Afaf Rashed

Tara Holman

Assistant Professor

Professor of Theoretical Mechanics

Karin Ljung


Auxin is a key hormone regulating plant growth and development. We combine experiments and mathematical modeling to reveal how auxin levels are maintained via feedback regulation of genes encoding key metabolic enzymes. We describe how regulation of auxin oxidation via transcriptional control of Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 (AtDAO1) expression is important at low to normal auxin concentrations. In contrast, higher auxin levels lead to increased Gretchen Hagen3 expression and auxin conjugation. Integrating this understanding into a multicellular model of root auxin dynamics successfully predicts that the dao1-1 mutant has an auxin-dependent longer root hair phenotype. Our findings reveal the importance of auxin homeostasis to maintain this hormone at optimal levels for plant growth and development.


Mellor, N. L., Band, L. R., Pěnčík, A., Novak, O., Rashed, A., Holman, T., …Owen, M. R. (2016). Dynamic regulation of auxin oxidase and conjugating enzymes AtDAO1 and GH3 modulates auxin homeostasis. Proceedings of the National Academy of Sciences, 113(39), 11022-11027.

Journal Article Type Article
Acceptance Date Jul 29, 2016
Online Publication Date Sep 20, 2016
Publication Date Sep 27, 2016
Deposit Date Sep 9, 2016
Publicly Available Date Mar 21, 2017
Journal Proceedings of the National Academy of Sciences
Print ISSN 0027-8424
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 113
Issue 39
Pages 11022-11027
Keywords Hormone Regulation, Auxin, Metabolism, Homeostasis, Arabidopsis Thaliana
Public URL
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