Creatine ingestion augments dietary carbohydrate mediated muscle glycogen supercomposition during the initial 24 hrs of recovery following prolonged exhaustive exercise in humans

Roberts, Paul A.; Fox, John; Peirce, Nicholas; Jones, Simon W.; Casey, Anna; Greenhaff, Paul L.

Abstract

Muscle glycogen availability can limit endurance exercise performance. We previously demonstrated 5 days of creatine (Cr) and carbohydrate (CHO) ingestion augmented post-exercise muscle glycogen storage compared to CHO feeding alone in healthy volunteers. Here we aimed to characterise the time-course of this Cr-induced response under more stringent and controlled experimental conditions and identify potential mechanisms underpinning this phenomenon. Fourteen healthy, male volunteers cycled to exhaustion at 70% VO2peak. Muscle biopsies were obtained at rest immediately post-exercise and after 1, 3 and 6 days of recovery, during which Cr or placebo supplements (20g.day-1) were ingested along with a prescribed high CHO diet (37.5 kcal.kg body mass-1.day-1, >80% calories CHO). Oral-glucose tolerance tests (oral-GTT) were performed pre-exercise and after 1, 3 and 6 days of Cr and placebo supplementation. Exercise depleted muscle glycogen content to the same extent in both treatment groups. Creatine supplementation increased muscle total-Cr, free-Cr and phosphocreatine (PCr) content above placebo following 1, 3 and 6 days of supplementation (all P<0.05). Creatine supplementation also increased muscle glycogen content noticeably above placebo after 1 day of supplementation (P<0.05), which was sustained thereafter. This study confirmed dietary Cr augments post-exercise muscle glycogen super-compensation, and demonstrates this occurred during the initial 24 h of post-exercise recovery (when muscle total-Cr had increased by <10%). This marked response ensued without apparent treatment differences in muscle insulin sensitivity (oral-GTT, muscle GLUT4 mRNA), osmotic stress (muscle c-fos and HSP72 mRNA) or muscle cell volume (muscle water content) responses, such that another mechanism must be causative.

Citation

Roberts, P. A., Fox, J., Peirce, N., Jones, S. W., Casey, A., & Greenhaff, P. L. (2016). Creatine ingestion augments dietary carbohydrate mediated muscle glycogen supercomposition during the initial 24 hrs of recovery following prolonged exhaustive exercise in humans. Amino Acids, 48(8), 1831–1842. https://doi.org/10.1007/s00726-016-2252-x

• Journal Article Type: Article
• Acceptance Date: May 3, 2016
• Online Publication Date: May 19, 2016
• Publication Date: 2016-08
• Deposit Date: May 20, 2016
• Publicly Available Date: May 20, 2016
• Journal: Amino Acids
• Print ISSN: 0939-4451
• Electronic ISSN: 1438-2199
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 48
• Issue: 8
• Pages: 1831–1842
• DOI: https://doi.org/10.1007/s00726-016-2252-x
• Keywords: Glycogen storage; Glucose tolerance; Phosphocreatine; Insulin sensitivity
• Public URL: https://nottingham-repository.worktribe.com/output/789612
• Publisher URL: http://link.springer.com/article/10.1007%2Fs00726-016-2252-x
• Contract Date: May 20, 2016