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Mapping polyclonal antibody responses to bacterial infection using next generation phage display

Naqid, Ibrahim A.; Owen, Jonathan P.; Maddison, Ben C.; Spiliotopoulos, Anastasios; Emes, Richard D.; Warry, Andrew; Tchorzewska, Monika; Martelli, Francesca; Gosling, Rebecca J.; Davies, Robert H.; La Ragione, Roberto M.; Gough, Kevin C.

Authors

Ibrahim A. Naqid

Jonathan P. Owen

Ben C. Maddison

Anastasios Spiliotopoulos

RICHARD EMES richard.emes@nottingham.ac.uk
Professor of Bioinformatics

Andrew Warry

Monika Tchorzewska

Francesca Martelli

Rebecca J. Gosling

Robert H. Davies

Roberto M. La Ragione

KEVIN GOUGH kevin.gough@nottingham.ac.uk
Professor of Biochemistry and Pathology



Abstract

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. In the first instance, pigs experimentally infected with Salmonella enterica serovar Typhimurium was investigated. IgG samples from twelve infected pigs were probed in parallel and phage binding compared to that with equivalent IgG from pre-infected animals. Seventy- seven peptide mimotopes were enriched specifically against sera from multiple infected animals. Twenty-seven of these peptides were tested in ELISA and twenty-two were highly discriminatory for sera taken from pigs post-infection (P < 0.05) indicating that these peptides are mimicking epitopes from the bacteria. In order to further test this methodology, it was applied to differentiate antibody responses in poultry to infections with distinct serovars of Salmonella enterica. Twenty-seven peptides were identified as being enriched specifically against IgY from multiple animals infected with S. Enteritidis compared to those infected with S. Hadar. Nine of fifteen peptides tested in ELISA were highly discriminatory for IgY following S. Enteritidis infection (p < 0.05) compared to infections with S. Hadar or S. Typhimurium.

Citation

Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., …Gough, K. C. (2016). Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Scientific Reports, 6, https://doi.org/10.1038/srep24232

Journal Article Type Article
Acceptance Date Mar 21, 2016
Online Publication Date Apr 13, 2016
Publication Date Apr 13, 2016
Deposit Date Jun 1, 2016
Publicly Available Date Jun 1, 2016
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 6
Article Number 24232
DOI https://doi.org/10.1038/srep24232
Public URL http://eprints.nottingham.ac.uk/id/eprint/33690
Publisher URL http://www.nature.com/articles/srep24232
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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