Skip to main content

Research Repository

Advanced Search

Controlling equine influenza: Traditional to next generation serological assays

Kinsley, Rebecca; Scott, Simon D.; Daly, Janet M.

Authors

Rebecca Kinsley

Simon D. Scott

JANET DALY janet.daly@nottingham.ac.uk
Professor of Viral Zoonoses



Abstract

Serological assays provide an indirect route for the recognition of infectious agents via the detection of antibodies against the infectious agent of interest within serum. Serological assays for equine influenza A virus can be applied for different purposes: diagnosing infections; subtyping isolates; surveillance of circulating strains; and to evaluate the efficacy of vaccines before they reach the market. Haemagglutination inhibition (HI) and single radial haemolysis (SRH) assays are most commonly used in the equine field. This review outlines how both these assays together with virus neutralization (VN) and ELISA are performed, interpreted and applied for the control of equine influenza, giving the limitations and advantages of each. The pseudotyped virus neutralization assay (PVNA) is also discussed as a promising prospect for the future of equine influenza virus serology.

Citation

Kinsley, R., Scott, S. D., & Daly, J. M. (2016). Controlling equine influenza: Traditional to next generation serological assays. Veterinary Microbiology, 187, 15-20. https://doi.org/10.1016/j.vetmic.2016.03.006

Journal Article Type Article
Acceptance Date Mar 8, 2016
Online Publication Date Mar 10, 2016
Publication Date May 1, 2016
Deposit Date Apr 28, 2016
Publicly Available Date Apr 28, 2016
Journal Veterinary Microbiology
Print ISSN 0378-1135
Electronic ISSN 0378-1135
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 187
Pages 15-20
DOI https://doi.org/10.1016/j.vetmic.2016.03.006
Keywords Equine influenza ; Serological assay ; Neutralization assay
Public URL http://eprints.nottingham.ac.uk/id/eprint/33013
Publisher URL http://www.sciencedirect.com/science/article/pii/S0378113516300591
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

Files


Controlling equine influenza.pdf (121 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





You might also like



Downloadable Citations