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Sequence and partial functional analysis of canine Bcl-2 family proteins

de Brot, Simone; Schade, Benjamin; Croci, Martina; Dettwiler, Martina; Guscetti, Franco

Authors

Simone de Brot simone.debrot@nottingham.ac.uk

Benjamin Schade Benjamin.Schade@tgd-bayern.de

Martina Croci marty_e_gatti@hotmail.com

Martina Dettwiler mdettwiler@gmail.com

Franco Guscetti gufo@vetpath.uzh.ch



Abstract

Dogs present with spontaneous neoplasms biologically similar to human cancers. Apoptotic pathways are deregulated during cancer genesis and progression and are important for therapy. We have assessed the degree of conservation of a set of canine Bcl-2 family members with the human and murine orthologs. To this end, seven complete canine open reading frames were cloned in this family, four of which are novel for the dog, their sequences were analyzed, and their functional interactions were studied in yeasts. We found a high degree of overall and domain sequence homology between canine and human proteins. It was slightly higher than between murine and human proteins. Functional interactions between canine pro-apoptotic Bax and Bak and anti-apoptotic Bcl-xL, Bcl-w, and Mcl-1 were recapitulated in yeasts. Our data provide support for the notion that systems based on canine-derived proteins might faithfully reproduce Bcl-2 family member interactions known from other species and establish the yeast as a useful tool for functional studies with canine proteins.

Citation

de Brot, S., Schade, B., Croci, M., Dettwiler, M., & Guscetti, F. (2016). Sequence and partial functional analysis of canine Bcl-2 family proteins. Research in Veterinary Science, 104, https://doi.org/10.1016/j.rvsc.2015.12.001

Journal Article Type Article
Acceptance Date Dec 4, 2015
Online Publication Date Dec 7, 2015
Publication Date Feb 28, 2016
Deposit Date Oct 31, 2016
Publicly Available Date Oct 31, 2016
Journal Research in Veterinary Science
Print ISSN 0034-5288
Electronic ISSN 1532-2661
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 104
DOI https://doi.org/10.1016/j.rvsc.2015.12.001
Keywords Bcl-2 family; Canine; Cloning; Comparative sequence analysis; Functional yeast study
Public URL http://eprints.nottingham.ac.uk/id/eprint/37341
Publisher URL http://www.sciencedirect.com/science/article/pii/S0034528815301028
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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