Bioaccessibility of carotenoids (β-carotene and lutein) from intact and disrupted microalgae (Chlamydomonas reinhardtii)

Akepach, Patchaniya; Ribeiro-Filho, Normando; Wattanakul, Jutarat; Darwish, Randa; Gedi, Mohamed A.; Gray, David A.

Abstract

Chlamydomonas reinhardtii (C. reinhardtii) has a potential as a novel source for food/feed because it contains several constituents including bioactive compounds. However, its multilayer cell wall (hydroxyproline-rich glycoprotein [HRPGs]) may restrict the bioaccessibility of its nutrients. Therefore, using disruption techniques such as hydrodynamic cavitation (HDC) can be useful for assessing single cell compounds. This work aims to evaluate the impact of HDC on the bioaccessibility of carotenoids (β-carotene and lutein) from C. reinhardtii. Our results illustrated that digestive enzymes cannot fully break down the cell walls beside HDC process generates their significant change. The intact C. reinhardtii (ICR) and disrupted C. reinhardtii (DCR) have a comparable lutein bioaccessibility, in contrast, DCR decreased the biocessibility of β-carotene. HDC decreased the biocessibility of β-carotene in the small intestine although 37% of total carotenoids from DRC were absorbed.

Citation

Akepach, P., Ribeiro-Filho, N., Wattanakul, J., Darwish, R., Gedi, M. A., & Gray, D. A. (2022). Bioaccessibility of carotenoids (β-carotene and lutein) from intact and disrupted microalgae (Chlamydomonas reinhardtii). LWT - Food Science and Technology, 160, Article 113292. https://doi.org/10.1016/j.lwt.2022.113292

• Journal Article Type: Article
• Acceptance Date: Feb 25, 2022
• Online Publication Date: Mar 12, 2022
• Publication Date: Apr 15, 2022
• Deposit Date: Oct 25, 2022
• Publicly Available Date: Oct 26, 2022
• Journal: LWT
• Print ISSN: 0023-6438
• Electronic ISSN: 0023-6438
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 160
• Article Number: 113292
• DOI: https://doi.org/10.1016/j.lwt.2022.113292
• Keywords: Food Science
• Public URL: https://nottingham-repository.worktribe.com/output/7611959
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0023643822002274?via%3Dihub