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Cell and protein compatible 3D bioprinting of mechanically strong constructs for bone repair

Sawkins, Michael John; Mistry, Pritesh; Brown, Bryan N.; Shakesheff, Kevin M.; Bonassar, Larry J.; Yang, Jing

Authors

Michael John Sawkins

Pritesh Mistry

Bryan N. Brown

Larry J. Bonassar

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JING YANG JING.YANG@NOTTINGHAM.AC.UK
Assistant Professor



Abstract

Rapid prototyping of bone tissue engineering constructs often utilizes elevated temperatures, organic solvents and/or UV light for materials processing. These harsh conditions may prevent the incorporation of cells and therapeutic proteins in the fabrication processes. Here we developed a method for using bioprinting to produce constructs from a thermoresponsive microparticulate material based on poly(lactic-co-glycolic acid) at ambient conditions. These constructs could be engineered with yield stresses of up to 1.22 MPa and Young’s moduli of up to 57.3 MPa which are within the range of properties of human cancellous bone. Further study showed that protein-releasing microspheres could be incorporated into the bioprinted constructs. The release of the model protein lysozyme from bioprinted constructs was sustainted for a period of 15 days and a high degree of protein activity could be measured up to day 9. This work suggests that bioprinting is a viable route to the production of mechanically strong constructs for bone repair under mild conditions which allow the inclusion of viable cells and active proteins.

Journal Article Type Article
Publication Date Jul 2, 2015
Journal Biofabrication
Print ISSN 1758-5082
Electronic ISSN 1758-5082
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 7
Issue 3
APA6 Citation Sawkins, M. J., Mistry, P., Brown, B. N., Shakesheff, K. M., Bonassar, L. J., & Yang, J. (2015). Cell and protein compatible 3D bioprinting of mechanically strong constructs for bone repair. Biofabrication, 7(3), doi:10.1088/1758-5090/7/3/035004
DOI https://doi.org/10.1088/1758-5090/7/3/035004
Publisher URL http://iopscience.iop.org/article/10.1088/1758-5090/7/3/035004
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information Accepted author manuscript. Copyright IOP Publishing.

Sawkins, M.J., Mistry, P., Brown, B.N., Shakesheff, K.M., Bonassar, L.J. and Yang, J. 2015. Cell and protein compatible 3D bioprinting of mechanically strong constructs for bone repair. Biofabrication. 7(035004)

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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