Dissecting the contribution of knee joint NGF to spinal nociceptive sensitization in a model of OA pain in the rat

Sagar, Devi Rani; Nwosu, Lilian Ngozi; Walsh, David A.; Chapman, Victoria

Abstract

Objective: Although analgesic approaches targeting nerve growth factor (NGF) for the treatment of osteoarthritis (OA) pain remain of clinical interest, neurophysiological mechanisms by which NGF contribute to OA pain remain unclear. We investigated the impact of local elevation of knee joint NGF on knee joint, vs remote (hindpaw), evoked responses of spinal neurones in a rodent model of OA pain.
Design: In vivo spinal electrophysiology was carried out in anaesthetised rats with established pain behaviour and joint pathology following intra-articular injection of monosodium iodoacetate (MIA), vs injection of saline. Neuronal responses to knee joint extension and flexion, mechanical punctate stimulation of the peripheral receptive fields over the knee and at a remote site (ipsilateral hind paw) were studied before, and following, intra-articular injection of NGF (10 mg/50 ml) or saline.
Results: MIA-injected rats exhibited significant local (knee joint) and remote (lowered hindpaw withdrawal thresholds) changes in pain behaviour, and joint pathology. Intra-articular injection of NGF significantly (P

Citation

Sagar, D. R., Nwosu, L. N., Walsh, D. A., & Chapman, V. (2015). Dissecting the contribution of knee joint NGF to spinal nociceptive sensitization in a model of OA pain in the rat. Osteoarthritis and Cartilage, 23(6), https://doi.org/10.1016/j.joca.2015.01.010

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2015
• Online Publication Date: Jan 23, 2015
• Publication Date: Jun 30, 2015
• Deposit Date: May 21, 2018
• Publicly Available Date: May 21, 2018
• Journal: Osteoarthritis and Cartilage
• Print ISSN: 1063-4584
• Electronic ISSN: 1522-9653
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 6
• DOI: https://doi.org/10.1016/j.joca.2015.01.010
• Public URL: https://nottingham-repository.worktribe.com/output/753518
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1063458415000230