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DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS

Glossop, John R.; Haworth, Kim E.; Emes, Richard D.; Nixon, Nicola B.; Packham, Jon C.; Dawes, Peter T.; Fryer, Anthony A.; Mattey, Derek L.; Farrell, William E.

Authors

John R. Glossop

Kim E. Haworth

Richard D. Emes

Nicola B. Nixon

Jon C. Packham

Peter T. Dawes

Anthony A. Fryer

Derek L. Mattey

William E. Farrell



Abstract

Aims: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). Herein, we performed genome-wide DNA methylation profiling of RA FLS derived from synovial fluid, a more readily accessible source of disease-associated cells.
Patients & Methods: Synovial fluid-derived FLS were isolated and expanded in vitro from 12 patients with RA and osteoarthritis (OA). DNA methylation was interrogated using HumanMethylation450 BeadChips and bisulfite pyrosequencing.
Results: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and OA fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (~40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes.
Conclusions: Synovial fluid-derived RA FLS show methylation changes across multiple genes. A subset of these are common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.

Journal Article Type Article
Publication Date Jun 1, 2015
Journal Epigenomics
Print ISSN 1750-1911
Electronic ISSN 1750-1911
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 7
Issue 4
APA6 Citation Glossop, J. R., Haworth, K. E., Emes, R. D., Nixon, N. B., Packham, J. C., Dawes, P. T., …Farrell, W. E. (2015). DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7(4), https://doi.org/10.2217/epi.15.15
DOI https://doi.org/10.2217/epi.15.15
Keywords Rheumatoid arthritis; DNA Methylation; Fibroblast-like synoviocyte; Synovial fluid; Epigenetics; Osteoarthritis
Publisher URL http://www.futuremedicine.com/doi/10.2217/epi.15.15
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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